Abstract: Objective:Optimized the purification process of polyphenols from Pinus sylvestris L. bark and made the best use of its polyphenols resource. Method:The adsorption rate and desorption rate were detection indicators. Optimum resin for purification of polyphenols was determined. As the index to recovery and purity of polyphenols, influential factors were successively investigated, and the response surface design was used to optimize this process. Result:The optimum conditions of purification were sample solution concentration 3. 0mg/mL, 30mL sample solutionin in pH 3. 5, feeding speed 5BV/h, eluting speed 4. 25BV/h and the concentration of ethanol 53%. Purity of Pinus sylvestris L. bark polyphenols was 67. 91% and the recovery was 68. 12%. Conclusion:Purity of pinus sylvestris L. bark polyphenols was from 37. 25% to 67. 91% according to this purification condition, which indicated that D101 macroporous resin had distinct effect on purification of polyphenols from pinus sylvestris L. var. mongolica barks.