紫苏秸秆纤维素酶水解工艺优化研究

Process optimization on cellulose enzymatic hydrolysis of Perilla frutescens stalk

摘要: 研究了紫苏秸秆酶法水解的最佳工艺条件。采用DNS法测定紫苏秸秆水解液中还原糖的含量,从而确定其酶水解率,对酶解时间﹑酶用量﹑pH3个因素进行单因素实验分析,再通过正交实验对酶解工艺进行优化。实验结果表明:最佳酶解工艺条件为酶解时间24h,pH5.0,纤维素酶用量720U,在此条件下,酶水解率可达72.42%。

Abstract: The process optimization on cellulose enzymatic hydrolysis of Perilla frutescens stalks was studied. The content of reducing sugar was determined by DNS method to evaluate the enzymatic hydrolysis.After analysis on hydrolysis time, enzyme content and pH value respectively, the optimized hydrolysis conditions were obtained by orthogonal experiments.Experimental results showed that the optimum condition was: reaction time 24h, enzyme content 720U, pH5.0.Under the optimum condition, the efficiency of enzymatic hydrolysis could reach 72.42%.