Abstract: Based on the investigation of the inclusion rate of cyclodextrin-menthol and inclusion thermodynamic, the effect of preparation and drying process on the inclusion effect were studied.Results showed that 1∶1 of inclusion was formed by β- cyclodextrin and menthol, the inclusion content was highest at 50℃.Collid mill method would cause better inclusion result, spray drying would cause serious lose of menthol.Vacuum drying were in favor of sustain the inclusion effect of supermolecular.This study can provide a reference and accordance for cyclodextrin- menthol complex industrialization.