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中国精品科技期刊2020
赖蓓蕾, 金寒冰, 方丽, 吴祥庭. 坛紫菜水溶膳食纤维二次正交旋转组合法提取工艺的优化及其抗油脂氧化研究[J]. 食品工业科技, 2014, (16): 206-211. DOI: 10.13386/j.issn1002-0306.2014.16.038
引用本文: 赖蓓蕾, 金寒冰, 方丽, 吴祥庭. 坛紫菜水溶膳食纤维二次正交旋转组合法提取工艺的优化及其抗油脂氧化研究[J]. 食品工业科技, 2014, (16): 206-211. DOI: 10.13386/j.issn1002-0306.2014.16.038
LAI Pei-lei, JIN Han-bing, FANG Li, WU Xiang-ting. Optimizing extraction of soluble dietary fiber from porphyra haitanensis through quadratic regression orthogonal design and its oil antioxidation[J]. Science and Technology of Food Industry, 2014, (16): 206-211. DOI: 10.13386/j.issn1002-0306.2014.16.038
Citation: LAI Pei-lei, JIN Han-bing, FANG Li, WU Xiang-ting. Optimizing extraction of soluble dietary fiber from porphyra haitanensis through quadratic regression orthogonal design and its oil antioxidation[J]. Science and Technology of Food Industry, 2014, (16): 206-211. DOI: 10.13386/j.issn1002-0306.2014.16.038

坛紫菜水溶膳食纤维二次正交旋转组合法提取工艺的优化及其抗油脂氧化研究

Optimizing extraction of soluble dietary fiber from porphyra haitanensis through quadratic regression orthogonal design and its oil antioxidation

  • 摘要: 以坛紫菜为原料,采用复合酶法提取水溶性膳食纤维,以猪油、菜油为材料考察其抗油脂氧化作用。在单因素实验基础上,采用二次正交旋转组合设计法对坛紫菜水溶性膳食纤维提取工艺进行优化,建立纤维素酶添加量(X1)、酶解pH(X2)、酶解温度(X3)和酶解时间(X4)等4个因素与水溶性膳食纤维提取率之间的回归模型。结果表明:纤维素酶法提取的最佳工艺参数为酶添加量1.1%、酶解pH5.8、酶解温度55℃和酶解时间1.5h,在此条件下坛紫菜水溶性膳食纤维提取率为9.80%±0.12%;膨胀力为1.97mL/g,持水力为276%,坛紫菜水溶性膳食纤维能明显减缓油脂POV值升高趋势,且具有一定的时间和浓度依赖性,显示坛紫菜可溶性膳食纤维具有一定的抗油脂氧化能力。 

     

    Abstract: Taking porphyra haitanensis as raw material, the composite enzymatic extraction technology was used to extract soluble dietary fiber, the antioxygenic activity of the soluble dietary fiber of porphyra haitanensis for lard and vegetable oilwas study. Based on single factor experiments, extraction conditions of soluble dietary fiber in porphyra haitanensis were optimized through quadratic regression orthogonal design. A quadratic regression orthogonal model was established to express the relationship between soluble dietary fiber yield (Y) and four factors including dosage of cellulase (X1) , enzymolysis pH (X2) , enzymolysis temperature (X3) , and enzymolysis time (X4) . Results showed :The optimal extraction paraneters were :dosage of cellulase 1.1%, enzymolysis pH5.8, enzymolysis temperature 55℃, and enzymolysis time 1.5h. Under such conditions, the highest yield of soluble dietary fiber, which was 9.80% ±0.12%. The expansive capacity and water holding capacity were 1.97mL/g and 276%, respectively. The soluble dietary fiber of porphyra haitanensis could significantly slow POV value rising trends of oils and fats, and had a certain amount of time and concentration dependent.Prompt with antioxidant activity of the soluble dietary fiber of porphyra haitanensis.

     

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