Abstract: Total phenols from Suaeda glauca Bge Seed growing on beach was extracted using ethanol as solvent by ultrasonic- assisted extraction ( UAE) method, and extraction process was optimized by Box- Behnken Design in response surface methodology ( RSM) based on single factor experiment, which investigated the influence of temperature, ultrasonic time, solid- liquid ratio and volume fraction of ethanol on total phenolic content ( TPC) . The regression model was built up and interactions between factors were analyzed. The results showed that optimal extraction conditions were 80℃, 80 min, 23g / m L of solid- liquid ratio and 80% of ethanol solvent, and regression model was obtained with 996.94μg / g of theory content of total phenols.Under the optimum conditions, the average value of TPC was 986.03μg / g, RSD% = 1.41.This indicated that the regression model was accurate and reliable for investigation on TPC.