Abstract: 20 bacterias were isolated from pckle during natural fermentation. 12 of them were gram-positive bacterias, 7 of these gram-positive bacterias were catalogued as Lactobacillus rhamnosus strains by testing producing lactic acid, the rate of producing the acid, the physiological and biochemical characteristics. It was also studied that the antimicrobial was assayed by the oxford cup and the anti-oxidation abilities were tesed by the scavenging the ·OH, DPPH· and O2·-of Rhamnose lactobacillus fermented liquid. The results showed as follows:these 7 Lactobacillus rhamnosus strains have antibacterial activity against Escherichia coli, Su Yunjun Bacillus and Staphylococcus aureus. Except LR6 Lactobacillus rhamnosus strain, they also could inhibit Bacillus subtillis, the Rhamnose lactobacillus fermented liquid could scaveng the ·OH, DPPH· and O2·-free radicals obviously.