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中国精品科技期刊2020
束文举, 何林霞, 蒋双双, 刘双玲, 郭进, 陆健, 高献礼. 甜油滋味物质及滋味特征研究[J]. 食品工业科技, 2015, (11): 54-58. DOI: 10.13386/j.issn1002-0306.2015.11.002
引用本文: 束文举, 何林霞, 蒋双双, 刘双玲, 郭进, 陆健, 高献礼. 甜油滋味物质及滋味特征研究[J]. 食品工业科技, 2015, (11): 54-58. DOI: 10.13386/j.issn1002-0306.2015.11.002
SHU Wen-ju, HE Lin-xia, JIANG Shuang-shuang, LIU Shuang-ling, GUO Jin, LU Jian, GAO Xian-li. Study on the characteristics of taste compounds and taste of sweet soy sauce[J]. Science and Technology of Food Industry, 2015, (11): 54-58. DOI: 10.13386/j.issn1002-0306.2015.11.002
Citation: SHU Wen-ju, HE Lin-xia, JIANG Shuang-shuang, LIU Shuang-ling, GUO Jin, LU Jian, GAO Xian-li. Study on the characteristics of taste compounds and taste of sweet soy sauce[J]. Science and Technology of Food Industry, 2015, (11): 54-58. DOI: 10.13386/j.issn1002-0306.2015.11.002

甜油滋味物质及滋味特征研究

Study on the characteristics of taste compounds and taste of sweet soy sauce

  • 摘要: 本文以生抽酱油为对照,通过常规指标分析法、十二烷基磺酸钠-聚丙烯酰胺凝胶电泳法(SDS-PAGE)、高效液相色谱法(HPLC)并结合感官评价对甜油的常规指标、蛋白质/多肽分子量分布、氨基酸组成和感官属性特征进行了分析。结果显示:甜油中Na Cl、总糖、还原糖、无盐固形物、总酸含量分别比生抽酱油高约28%、172%、176%、120%、58%,而其氨基酸态氮含量显著低于生抽酱油(约35%)。甜油中高分子量蛋白质/多肽的比例和总含量显著高于生抽酱油。甜油中游离氨基酸以甜味氨基酸(45%)为主,其次为苦味氨基酸和鲜味氨基酸;而生抽酱油以鲜味氨基酸(57%)为主,其次为苦味氨基酸和甜味氨基酸。感官评价结果证实了甜油咸、甜突出,鲜、酸、苦诸味协调。 

     

    Abstract: In this paper, light soy sauce was used as control, the characteristics of proximate indices, molecular weight distribution of protein / polypeptide, amino acid composition and sensory attributes of sweet soy sauce were studied using proximate indices analysis, sodium dodecyl sulfate- polyacrylamide gel electrophoresis, high performance liquid chromatography in combination with sensory evaluation. Results showed that the contents of Na Cl, total sugar, reducing sugar, salt- free solid and total acid in sweet soy sauce were 28%, 172%, 176%, 120%and 58% higher than those in light soy sauce, however its amino acid nitrogen was approximate 35% lower than the control.The proportion and the content of protein / polypeptide with high molecular weight in sweet soy sauce were remarkably higher than those in the control. The amino acid composition of sweet soy sauce was characterized by a large amount of sweet amino acids ( 45%) , followed by bitter amino acids and umami amino acids; however, the amino acid composition of the control was characterized by dominant umami amino acids ( 57%) , followed by bitter amino acids and sweet amino acids.Sensory evaluation indicated that sweet soy sauce processed prominent tastes of salty and sweet, and a harmonious mixture of umami, sour and bitter, etc.

     

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