Abstract: The investigation on the adsorption of whey protein isolate( WPI) onto different calcium particles,including calcium carbonate,hydroxyapatite and tricalcium phosphate,were carried out via different characterizations including ζ- potential,confocal laser scanning microscope,adsorption isotherms,preference adsorption and suspension stability. The surface charge of calcium particles were increased after WPI was adsorbed. Different adsorption behaviors were concluded for different calcium particles according to the calculated adsorption isotherms.β- Lactoglobulin was observed to be preferentially adsorbed on to the particle surface compared withα-lactalbumin. The stability of the insoluble calcium salt suspension were improved with WPI adsorbed,which was a great significance for the improvement of calcium fortified milk or beverage.