鲜切生菜致腐霉菌的分离鉴定

Isolation and identification of spoilage molds from fresh- cut lettuce

摘要: 对引起鲜切生菜腐败的霉菌进行分离鉴定。通过形态学鉴定方法,从鲜切生菜中分离出46株霉菌,其中青霉菌属(Penicillium)28株,曲霉属(Aspergillus)13株,枝孢属(Cladosporium)3株,镰孢属(Fusarium)2株。根据不同属在不同培养基上的性状,通过在察氏琼脂培养基(CA)、察氏酵母膏琼脂培养基(CYA)、查氏酵母浸出液加蔗糖培养基(CY20S)、25%甘油硝酸盐琼脂培养基(G25N)和玉米粉琼脂培养基(CMA)等鉴定培养基上的特征及显微镜观察,将28株青霉菌属鉴定为三种:橘灰青霉(P.aurantiogriseum)、鲜绿青霉(P.viridicatum)、草酸青霉(P.oxalicum)。13株曲霉属鉴定为两种:泡盛曲霉(A.awamori)、蜂蜜曲霉(A.melleus)。枝孢属鉴定为一种:枝孢样枝孢霉(C.cladosporioides)。

Abstract: The spoilage molds which cause fresh- cut lettuce corruption were identified.According to morphological character method,46 strains of molds were isolated from fresh- cut lettuce,in which there were 28 Penicillium strains,13 Aspergillus strains,3 Cladosporium strains and 2 Fusarium strains.The different molds were identified by the cultivation characters and microscopic examination in different medium including CA,CYA,CY20 S,G25N,and CMA.The results showed that 3 kinds of Penicillium were identified,including P.aurantiogriseum,P.viridicatum,P.oxalicum.2 kinds of Aspergillus were A.awamori and A.melleus.1 kind of Cladosporium was C.cladosporioides.