Abstract: In this paper,the main content of active components in Ginkgo biloba leaves was measured and the effect of its extract of different concentrations on the quality of pomfret( Pampus argenteus) during ice storage was investigated.Active substances from Ginkgo biloba leaves were extracted by ultrasonic- assisted method,the contents of total falconoid,total sugar and total phenol were determined respectively. Sensory evaluation,aerobic plate count( APC),K value,total volatile basis nitrogen( TVB- N),thiobarbituric acid( TBA) and p H were used to evaluate the quality of Pampus argenteus.The results showed that the contents of total flavonoids,total sugar and total phenol were 8.075 ± 0.021,79.360 ± 0.045 and 38.389 ± 0.034 mg / g respectively. Ginkgo biloba leaves extract could inhibit the growth of bacteria and the speed of lipid oxidation,slow down the increase of K value,TVB- N value,p H value and improve the shelf- life of Pampus argenteus. When compared with the controlled group,the samples with Ginkgo biloba leaves extract could prolong the shelf-life of Pampus argenteus to 15 d and 2.5 mg / m L of Ginkgo biloba leaves extract was the best for the preservation of Pampus argenteus during ice storage.