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中国精品科技期刊2020
陈虹霞, 王成章, 叶建中, 周昊, 陶冉, 李文君. 漆树籽粕多糖的提取工艺优化及抗氧化活性研究[J]. 食品工业科技, 2016, (04): 254-258. DOI: 10.13386/j.issn1002-0306.2016.04.043
引用本文: 陈虹霞, 王成章, 叶建中, 周昊, 陶冉, 李文君. 漆树籽粕多糖的提取工艺优化及抗氧化活性研究[J]. 食品工业科技, 2016, (04): 254-258. DOI: 10.13386/j.issn1002-0306.2016.04.043
CHEN Hong-xia, WANG Cheng-zhang, YE Jian-zhong, ZHOU Hao, TAO Ran, LI Wen-jun. Extraction optimization of crude polysaccharides from the seed cakes of Rhus succedanea L and its antioxidant activity[J]. Science and Technology of Food Industry, 2016, (04): 254-258. DOI: 10.13386/j.issn1002-0306.2016.04.043
Citation: CHEN Hong-xia, WANG Cheng-zhang, YE Jian-zhong, ZHOU Hao, TAO Ran, LI Wen-jun. Extraction optimization of crude polysaccharides from the seed cakes of Rhus succedanea L and its antioxidant activity[J]. Science and Technology of Food Industry, 2016, (04): 254-258. DOI: 10.13386/j.issn1002-0306.2016.04.043

漆树籽粕多糖的提取工艺优化及抗氧化活性研究

Extraction optimization of crude polysaccharides from the seed cakes of Rhus succedanea L and its antioxidant activity

  • 摘要: 以脱脂后的漆树籽粕为原料,采用热回流提取漆树籽粕多糖,通过单因素实验和正交实验优化该方法的最佳工艺条件,并进一步研究漆树籽粕多糖对羟自由基和ABTS+自由基的清除能力。结果表明:最佳工艺条件为提取时间2.5 h,料液比1∶20(g∶m L),提取温度80℃和提取次数2次,此时籽粕多糖的得率为1.561%。漆树籽粕多糖对清除羟自由基和ABTS+自由基的IC50分别为8.515 mg·m L-1和7.03 mg·m L-1,当漆树籽粕多糖的浓度为25 mg·m L-1时,对羟自由基的清除率达到61.5%,当浓度为10 mg·m L-1时,对ABTS+自由基的清除率达到58.4%。体外抗氧化实验表明漆树籽粕多糖抗氧化活性作用明显。 

     

    Abstract: Single factor and orthogonal methodology were used to optimize the extraction conditions of crude polysaccharides from the seed cakes of Rhus succedanea L,then the antioxidant(scavenging hydroxyl and ABTS radicals) of the crude polysaccharides from the seed cakes of Rhus succedanea L were possessed. The optimum extraction conditions were as follows :extraction time 2.5 h,ratio of material to water 1 ∶20(g∶m L),extraction temperature 80 ℃,and extraction times 2. Under the optimized conditions,an experimental yield was 1. 561 %. The IC_(50) values scavenging hydroxyl and ABTS~+radicals of the crude polysaccharides were8.515 mg·m L~(-1) and 7.03 mg·m L~(-1),respectively. The hydroxyl radical scavenging ability of polysaccharides reached 61.5% at 25 mg·m L~(-1). The ABTS+radicals scavenging ability of polysaccharides reached 58.4% at10 mg·m L~(-1). The seed cakes polysaccharides displayed significant antioxidant.

     

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