Abstract: In order to investigated antimicrobial activity of solid chlorine dioxide preservative on miliang kiwifruit,pathogenic fungi were isolated from diseased tissue by PDA plate. Using injury and no injury inoculation to screening strongest virulence strains. Morphological and molecular way were used to identify strains. The antimicrobial activity of solid ClO_2 preservative on the most virulent strains was investigated by using the method of mycelium growth rate. Research had revealed that seven pathogen strains were parted from rotten miliang kiwifruit. Three of them were deemed as the most virulent strains and identified as Myrothecium sp.,Phoma sp.and Alternaria sp. All three strains were inhibited by solid ClO_2 preservative under room temperature but couldn't completely inhibit their growth. The sequence of inhibition was Alternaria sp.,Myrothecium sp. and Phoma sp. The three strains were almost inhibited by solid ClO_2 preservative under 2 ℃. Solid ClO_2 spoilage of miliang kiwifruit had a good antibacterial effect. It can be used as a preservative for reducing the rate of corruption.