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中国精品科技期刊2020
邵双双, 贺亮, 韦朝阳, 李卫旗. 分心木酸性多糖SJP-2的理化性质及其抗氧化活性研究[J]. 食品工业科技, 2016, (05): 59-63. DOI: 10.13386/j.issn1002-0306.2016.05.003
引用本文: 邵双双, 贺亮, 韦朝阳, 李卫旗. 分心木酸性多糖SJP-2的理化性质及其抗氧化活性研究[J]. 食品工业科技, 2016, (05): 59-63. DOI: 10.13386/j.issn1002-0306.2016.05.003
SHAO Shuang- shuang, HE Liang, WEI Chao-yang, LI Wei-qi. Physicochemical properties and antioxidant activity of acid polysaccharide sjp-2 from Semen Juglandis[J]. Science and Technology of Food Industry, 2016, (05): 59-63. DOI: 10.13386/j.issn1002-0306.2016.05.003
Citation: SHAO Shuang- shuang, HE Liang, WEI Chao-yang, LI Wei-qi. Physicochemical properties and antioxidant activity of acid polysaccharide sjp-2 from Semen Juglandis[J]. Science and Technology of Food Industry, 2016, (05): 59-63. DOI: 10.13386/j.issn1002-0306.2016.05.003

分心木酸性多糖SJP-2的理化性质及其抗氧化活性研究

Physicochemical properties and antioxidant activity of acid polysaccharide sjp-2 from Semen Juglandis

  • 摘要: 以分心木为原料进行热水浸提,经过醇沉、脱色素、脱蛋白以及DEAE-Sepharose Fast Flow离子交换柱色谱和Sephadex-G50凝胶柱色谱分离纯化得到一种酸性多糖组分(SJP-2),再对该组分进行理化性质及抗氧化活性分析。结果表明:SJP-2不含蛋白,是一个低分散度,分子量较为均一的多糖,分子量为3.239×103g/mol;HPLC分析得知SJP-2为一种酸性多糖,其单糖组成及摩尔比例为阿拉伯糖∶半乳糖∶葡萄糖∶半乳糖醛酸=1∶2.62∶4.45∶18.53;抗氧化活性测得SJP-2清除DPPH自由基、羟自由基以及ABTS自由基的IC50分别为0.094、0.945、0.591 mg/m L,还原能力在1.0 mg/m L时达到1.158,ORAC值为(1217.99±31.22)μmol TE/g,说明SJP-2具有较高的体外抗氧化能力。 

     

    Abstract: The polysaccharide SJP- 2 from Semen Juglandis was obtained by hot water extraction and alcohol precipitation,and purified using DEAE- Sepharose Fast Flow chromatography and Sephadex- G50 gel column chromatography.The physicochemical properties and antioxidant activity of SJP- 2 were determined. The result showed that SJP- 2 without proteins,was a lower dispersion polysaccharide with uniform molecular of 3.239 ×10~3g / mol,and the purity was 99.61%.The SJP-2 was acid polysaccharide determined by HPLC,and composed of arabinose,galactose,glucose,and galacturonic acid with a molar ratio of 1 ∶ 2.62 ∶ 4.45 ∶ 18.53. The IC50 of DPPH scavenging activity,hydroxyl radical scavenging activity,and ABTS scavenging activity were 0.094,0.945,0.591 mg / m L,respectively. At 1 mg / m L,the reducing power was 1.158. The ORAC of SJP- 2 was( 1217.99 ±31.22) μmol TE / g.It was demonstrated that SJP-2 had higher antioxidant activity.

     

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