乳杆菌产抑菌活性物质与酵母菌DNA相互作用研究
Study on unteraction mechanism of antiyeast substance produced by lactobacillus and the yeast DNA
-
摘要: 本研究以具有抑酵母活性的乳杆菌ALAC-3和ALAC-4为研究对象,以白假丝酵母为指示菌,通过紫外光谱和荧光光谱法研究了乳杆菌所产抑菌活性物质与酵母菌DNA的相互作用机制。结果表明:酵母菌DNA与乳杆菌所产抑菌活性物质反应后,其紫外吸收值减小,产生了减色效应;随着作用时间的延长,紫外吸收值增加,呈现增色效应。加入抑菌物质后,酵母菌DNA荧光强度降低。抑菌物质与EB-DNA复合体系反应后荧光强度降低,EB与抑菌物质-DNA复合体系反应后荧光强度增加,说明溶液体系存在抑菌物质-DNA与EB-DNA两个复合体系竞争。Abstract: Two strains of lactobacillus ALAC- 3 and ALAC- 4 being of strong antiyeast ability were studied. Taking Candida albicans as the indicator strain,the interaction of antiyeast substance produced by lactobacillus and yeast DNA was investigated by ultraviolet spectrometry and fluorescence spectrometry. The results showed that the ultraviolet absorption value was decreased significantly and hypochromism were observed after adding anti-bacterial substances.With the extension of time,the ultraviolet absorption value was increased and hyperchromism were observed. The fluorescence intensity was decreased after adding anti- bacterial substances. When anti-bacterial substances and EB- DNA was reacted with each other,the fluorescence intensity was decreased significantly. And the fluorescence intensity was increased after EB and anti- bacterial- DNA was reacted. It indicated that there was the competition of anti- bacterial- DNA and EB- DNA in the solution system.