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中国精品科技期刊2020
王相伟, 邢翔, 马庆林. 过量表达葡萄糖脱氢酶对E.coli羟基脂肪酸合成能力的影响[J]. 食品工业科技, 2016, (05): 163-166. DOI: 10.13386/j.issn1002-0306.2016.05.023
引用本文: 王相伟, 邢翔, 马庆林. 过量表达葡萄糖脱氢酶对E.coli羟基脂肪酸合成能力的影响[J]. 食品工业科技, 2016, (05): 163-166. DOI: 10.13386/j.issn1002-0306.2016.05.023
WANG Xiang- wei, XING Xiang, MA Qing- lin. Effect of overexpression of glucose dehydrogenase on hydroxyfatty acids production of Escherichia coli[J]. Science and Technology of Food Industry, 2016, (05): 163-166. DOI: 10.13386/j.issn1002-0306.2016.05.023
Citation: WANG Xiang- wei, XING Xiang, MA Qing- lin. Effect of overexpression of glucose dehydrogenase on hydroxyfatty acids production of Escherichia coli[J]. Science and Technology of Food Industry, 2016, (05): 163-166. DOI: 10.13386/j.issn1002-0306.2016.05.023

过量表达葡萄糖脱氢酶对E.coli羟基脂肪酸合成能力的影响

Effect of overexpression of glucose dehydrogenase on hydroxyfatty acids production of Escherichia coli

  • 摘要: 在工程大肠杆菌生产羟基脂肪酸的基础上,为解决构建的以葡萄糖为底物合成羟基脂肪酸(HFAs)代谢途径中存在的细胞内还原力(NADPH)不平衡的问题,克隆了来源于巨大芽孢杆菌葡萄糖脱氢酶(GDH)基因,构建了胞内HFAs和NADPH联产的工程菌株;实验结果表明该重组大肠杆菌合成HFAs的产量得到显著提高,摇瓶条件下HFAs产量达到173.9 mg/L。具有较好的工业化开发前景。 

     

    Abstract: A metabolic pathway to synthesize hydroxyfatty acids( HFAs) from glucose was constructed. But the reducibility( NADPH) imbalance hampers its application. In order to increase the yield of HFAs,the glucose dehydrogenase( GDH) from Bacillus megaterium was cloned and a NADPH and HFAs co- product strains was built to solve the reducibility imbalance in cell. Under shake- flask conditions,this recombinant strain produced173.9 mg / L HFAs.The research laid the foundation in industrial applications for obtaining engineered Escherichia coli to produce HFAs with high production.

     

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