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中国精品科技期刊2020
何桂强, 冯笑, 吴重德, 黄钧, 周荣清. 多重胁迫对嗜盐四联球菌CGMCC 3792存活率及细胞成分的影响[J]. 食品工业科技, 2016, (05): 182-186. DOI: 10.13386/j.issn1002-0306.2016.05.027
引用本文: 何桂强, 冯笑, 吴重德, 黄钧, 周荣清. 多重胁迫对嗜盐四联球菌CGMCC 3792存活率及细胞成分的影响[J]. 食品工业科技, 2016, (05): 182-186. DOI: 10.13386/j.issn1002-0306.2016.05.027
HE Gui- qiang, FENG Xiao, WU Chong- de, HUANG Jun, ZHOU Rong-qing. Influence of multiple stresses on the survival and cellar constituent of Tetragenococcus halophilus CGMCC 3792[J]. Science and Technology of Food Industry, 2016, (05): 182-186. DOI: 10.13386/j.issn1002-0306.2016.05.027
Citation: HE Gui- qiang, FENG Xiao, WU Chong- de, HUANG Jun, ZHOU Rong-qing. Influence of multiple stresses on the survival and cellar constituent of Tetragenococcus halophilus CGMCC 3792[J]. Science and Technology of Food Industry, 2016, (05): 182-186. DOI: 10.13386/j.issn1002-0306.2016.05.027

多重胁迫对嗜盐四联球菌CGMCC 3792存活率及细胞成分的影响

Influence of multiple stresses on the survival and cellar constituent of Tetragenococcus halophilus CGMCC 3792

  • 摘要: 本研究以嗜盐四联球菌(Tetragenococcus halophilus CGMCC 3792)为实验菌株,考察了单因素(酸、热、盐)胁迫和交互保护对其存活的影响。结果表明,T.halophilus CGMCC 3792在p H4.0和45℃预适应处理60 min后细胞对酸致死胁迫(p H2.5,60 min)的存活率分别提高了10.6倍和8.8倍。此外,还考察了酸胁迫对细胞膜不饱和脂肪酸和胞内氨基酸含量的影响,结果表明,酸预适应后再经酸致死胁迫导致细胞膜不饱和脂肪酸18∶1n-9和18∶2n-6,9含量增加,分别是直接经酸致死胁迫的2.3倍和2.0倍;同时,分析胞内氨基酸含量,胞内谷氨酸、天冬氨酸、亮氨酸和异亮氨酸浓度分别是直接经酸致死胁迫的2.5倍、2.4倍、2.2倍和2.7倍。研究结果将有助于进一步解析嗜盐四联球菌环境胁迫抗性的生理机制。 

     

    Abstract: In this study,the influence ofsingle- stress( acid,heat,or salt) and cross- protection on the survival of Tetragenococcus halophilus CGMCC 3792 was investigated. The results indicated that cells were pre- adapted at p H4.0 or 45 ℃ for 60 min,and then acid challenged at p H2.5 for 60 min,and the survival rate increased 10.6- fold and 8.8- fold,respectively. Analysis of membrane fatty acids and intracellular amino acids in acid- adapted cells showed that unsaturated fatty acids18∶ 1n- 9 and 18 ∶ 2n- 6,9 were 2.3- fold and 2.0- fold higher than that in cells directly subjected to lethal acid stress,respectively. In addition,the intracellular concentrations of glutamate,aspartate,leucine,and isoleucinewere 2.5- fold,2.4- fold,2.2- fold and 2.7- fold higher than those in control cells,respectively. Results presented in this study may contribute to further elucidate the physiological mechanisms employed by T. halophilus during environmental stresses.

     

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