Abstract: A screening database for 29 kinds of antibacterial drugs including sulfonamides,fluoroquinolones,macrolides and lincosamides in meat was established by ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry. Combined with a rapid high throughput pretreatment,it can fast screen and test the antibacterial drugs in pork.Samples were extracted by Mcllvaine buffer and cleaned up by HLB solid phase extraction tube.Chromatography method was separated by BEH C₁₈ UPLC column,eluted whit gradient solvent,detected by MS^E acquisition and quantitative by parent ion with exact mass. The result showed that the LOQs of 29 antibacterial drugs in matrixes were between 0.5 μg/kg and 10 μg/kg.Average recoveries in 3 different spiked levels were 50.10% ~118.33%,RSD were 0.31% ~14.90%.This method can achieve a high throughput,multicomponents,rapid and open screening and appropriate in rapid screening antibacterial drugs in pork.