响应面优化石花菜琼胶提取工艺

Optimization of extraction process of Gelidium amansii agar by response surface methodology

摘要: 采用高温高压法提取石花菜琼胶,以提取率和凝胶强度为指标研究其提取工艺。在单因素实验的基础上,采用BBD中心组合实验设计,利用Design-Expert软件,建立数学模型,确定琼胶最佳提取工艺参数:柠檬酸为0.03%、提取时间为33 min,料液比为1∶36、提取温度为110℃,此条件下琼胶提取率高达37.85%,其感官性质、理化性质符合国家标准,凝胶强度为873 g/cm2,属于高强度产品,为石花菜深加工提供一定的理论依据。

Abstract: In this paper,high temperature and high pressure were used to extract Gelidium amansii agar.Extraction rate and gel strength were used as index of extraction process. Based on single factor experiments,BBD central composite design and Design- Expert software were used to build a mathematical model.The optimum extraction parameters of agar were determined as follows: citric acid 0.03%,extraction time 33 min,solid- liquid ratio 1 ∶ 36,extraction temperature 110 ℃.Under these conditions,the extraction rate of agar was 37.85%.Agar's organoleptic properties,physical and chemical properties met the standard.The strength of gel was 873 g / cm~2,which belonged to a high strength products for the industry. The extraction rate of high- quality agar was improved,and research base was provided.