Abstract: Study on optimum conditions of the purification of total flavonoids from the seeds of Ziziphus jujuba Mill.with macroporous adsorption resin. Meanwhile,to analyze the in vitro antioxidant capacities of the flavonoids. Five different polarity macroporous adsorption resin were selected by using static adsorption and dynamic adsorption tests,and the total flavonoids concentration of sample,sample volume,the volume fraction of the elution,the volume of elution and the flow rate were investigated,respectively.The flavonoids were evaluated by three models of antioxidant activity: DPPH·,ABTS+·and K3Fe( CN)6with VCas the positive control.The results indicated that AB-8macroporous adsorption resin was selected as the purification of the total flavonoids of Ziziphus jujuba Mill. The optimum technology conditions were as follows: the total flavonoids concentration of sample was 1.99 mg / m L,sample volume was 50 m L,the volume fraction of the elution was 50% ethanol,the volume of elution was 50 m L,and the flow rate was 1 m L / min after purification. Compared with VC,the flavonoids from Ziziphus jujuba Mill.had stronger capacity of scavenging on DPPH·,ABTS+·( IC500.70 mg / m L,0.15 mg / m L) and showed a high reduction capacity.