Abstract: Salmonella is an important zoonotic pathogens. This study collected 292 healthy yak and cattle samples in Sichuan Northwest plateau for Salmonella separation and of which antibiotic resistance research. The results showed that 15 plants Salmonella( 5 serovars) were isolated in yak which had no diarrhea symptoms samples,and the total detection rate was 7.73%,and the detection rate in fecal was 9.23%,muscle was 4.44%,liver was 11.86% respectively.12 plants Salmonella( 4 serovars)were isolated in cattle which had no diarrhea symptoms samples,and total detection rate was 12.24%,the detection rate in fecal was 22.86%,muscle was 2.70%,liver was 11.54% respectively. In the antibiotic susceptibility test,the resistance of yak Salmonella was less than cattle.However,yak and cattle Salmonella were showed high resistance to tetracycline,lincomycin and midecamycin.The yak Salmonella infection and antibiotic resistance were lower than cattle.