响应面优化超声辅助提取扫帚菜多糖工艺及其抗氧化性能
Optimization of ultrasonic-assisted extracting technology of polysaccharides from Kochia Scoparia (Linn.) Schrad by response surface methodology and its antioxidant properties
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摘要: 以扫帚菜为原料,对影响超声波辅助提取扫帚菜多糖工艺进行单因素实验,并对各影响因素进行BoxBehnken实验设计和响应面优化,得到了二次多项式回归方程,确定了最佳的提取工艺。并分析比较了扫帚菜多糖、抗坏血酸(VC)和特丁基对苯二酚(TBHQ)对1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除作用。结果表明,最佳提取工艺为液料比21 m L/g,超声功率76 W,超声温度72℃和超声时间24 min,在该条件下扫帚菜多糖得率为58.45 mg/g,与预测值相比,其相对误差为1.15%,说明该模型有效。扫帚菜多糖对DPPH自由基有较好的清除作用,具有一定的抗氧化能力,扫帚菜多糖,VC和TBHQ的抗氧化能力顺序为VC>扫帚菜多糖>TBHQ。Abstract: The ultrasonic-assisted extracting technology of polysaccharides from Kochia Scoparia ( Linn.) Schrad was optimized by response surface methodology. Based on the single factor experiment, a quadratic polynomial regression model and the optimal extraction conditions was established about the dependent variable ( extraction yield of polysaccharide) and independent variables ( liquid-material ratio, ultrasonic power, ultrasonic temperature and ultrasonic time) through a four factors-three levels Box-Benhnken design and response surface methodology. The scavenging effect of polysaccharides on 2, 2-diphenyl-1-picrylhydrazyl ( DPPH) free radical was also studied and comparing with ascorbic acid ( VC) and tertiary butylhydroquinone ( TBHQ) .The results showed that the optimum conditions of ultrasonic-assisted extraction of polysaccharides from Kochia Scoparia ( Linn.) Schrad were as follow: liquid-material ratio 21 m L/g, ultrasonic power 76 W, ultrasonic temperature 72 ℃ and ultrasonic time 24 min and yield of polysaccharide could reach up to 58.45 mg/g, the relative error was 1.15% compared to the predictive value, which indicated the feasible model fitted well with the experimental data. The Kochia Scoparia ( Linn.) Schrad polysaccharides could effectively eliminate the DPPH free radical, indicating that the polysaccharides had a certain antioxidant capacity.The antioxidant capacity followed the order, VC> polysaccharides > TBHQ.