Abstract:
To investigate the antioxidant activity of isochlorogenic acid A in vitro and in vivo. Isochlorogenic acid A from honeysuckle herbs was extracted.The antioxidant activities of isochlorogenic acid A was evaluated by spectrophotometry on its ability of scavenging ·OH, O
-2·, DPPH· and the reduction force.The zebrafish was used to test the antioxidant activities of isochlorogenic acid A in vivo.The result showed that the isochlorogenic acid A had well scavenging activity to DPPH, ·OH and O
-2·in the range of experimental concentration, the highest scavenging of DPPH and OH were reached 97.04%, 60.01%respectively.However, the highest scavenging of O
-2· reached just 28.04%. Determination of OD in reduction ability of isochlorogenic acid A up to 0.98, illustrating that isochlorogenic acid A had a highly reduction ability. In vivo experiments in zebrafish showed that the antioxidant capacities were reached to 60.4%, when the concentration of sample were 25 μg/m L. So the isochlorogenic acid A showed effective antioxidative action both in vitro and in vivo.
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