Abstract: To solve the problem of large temperature fluctuation and short cold-keeping time for aquatic products, the fresh ice formula of partial-frozen for aquatic products, such as sodium polyacrylate, sodium chloride and propanediol, were optimized on the basis of single factor experiments at the point of-3 ~-1.5 ℃. Box-Behnken experimental design and response surface methodology (RSM) were used to find the optimal formula. The results showed that the optimal fresh ice formula was 1.0%sodium polyacrylate, 0.6% sodium chloride and 2.1% propanediol, which could keep the temperature of partial-frozen (-3 ~-1.5 ℃) of aquatic for 130 min. Compared with traditional fresh ice of sodium chloride, complex fresh ice could prolong for about 60 min.Therefore, fresh ice formula could keep longer low-temperature time, make the temperature more stable and be helpful for the storage and processing of aquatic products.