Abstract: The chemical constituents of the seeds of Abelmoschus manihot ( L.) Medic. were determined by GC-MS, UV-Vis spectrophotpmetry, Weende, BCA and UPLC-TQ/MS. The seed of A. manihot was rich in fatty acids, soluble polysaccharides, celluloses, soluble protein, amino acids and nucleosides.Total content of the fatty acids ( palmitoleic acid, palmitic acid, linolic acid, oleic acid, linolenic acid, stearic acid) was 55.47 ¹02.17 mg/g, and the unsaturated fatty acids accounted for 78.01% ⁷9.40% of all these fatty acids. The contents of soluble polysaccharides, celluloses and soluble protein were 6.53% ~ 6.68%, 12.77% ~ 14.26%, and 10.36% ¹4.51%, respectively. Total of 19 amino acids and 7 nucleosides were detected in the seed, and the content of amino acids were 100.82 ¹01.51 mg/g, in which essential amino acids were accounted for 25%.The content of nucleosides in the seed was determined as 3.01 ~ 3.11 mg/g. The results provided a theoretical basis for the resourceful utilization of the seed of A.manihot.