Abstract: A method was established for determining acetamiprid, imidacloprid, carbendazim, pyrimethanil, difenoconazole residues in wild vegetables by ultra-high performance liquid chromatography-mass spectrometry ( ACQUITY UPLC/Xevo TQ-S) . Residues in samples were extracted with acetonitrile, and separated by C18 column with gradient elution using a mobile phase made up of acetonitrile and 0.1% formic acid ammonium acetate and detected. The results showed the linear range of0.0005².0000 mg/L with the correlation coefficients from 0.9947 to 0.9999.The recoveries rates at 0.005⁰.200 mg/kg levels were in the range of 73.43% ¹15.84%.The relative standard deviation ( RSD) was 2.04% ¹8.19%.The limits of detection were0.020⁰.510 μg/kg and the limits of quantitation were 0.066¹.699 μg/kg.The method proved to be simple, sensitive, rapid, accurate and precise for the determination of five kinds of pesticide residues in wild vegetables.