Abstract: The study aims to prepare and characterize spinosin phospholipid complex(SPI-PLC), which laid theoretical foundation for the development of a new type of natural sedative-hypnotic medicine and industrial production. Solvent evaporation method was used to prepare SPI-PLC, and complex yield ratio(CR)was served as evaluation parameter. Single factor experiments and Box-Behnken design were adopted to optimize the preparation conditions. Ultraviolet spectrum(UV)and infrared spectrum(IR)were used for analyzing the products, and the oil-water partition coefficient, particle size and dispersion of SPI-PLC were investigated. The results showed that solvent evaporation preparation was set up and its best preparation conditions were listed as follows:acetone as reaction dissolvants, the ratio of drug and phospholipid was 1:16.42, the rate of agitation, reaction temperature and time were 836 r/min, 40℃ and 3.27 h respectively. The compound rate reached up to 90.75%±0.64% under such circumstances. UV and IR were showed that the new SPI-PLC was formatted. The particle size and the polydispersity index(PDI)for the optimized SPI-PLC were(542.1±12.5) nm and 0.167±0.024. The apparent oil-water partition coefficient of SPI-PLC increased, which indicated that it had certain solubilization. Therefore, SPI-PLC was developed successfully using the simple preparation protocol, which provided the reference for the further research of spinosin.