Abstract: Objective:To study the in vitro antioxidant activities and anti-fatigue effect of chicory polysaccharide. Methods:The scavenging activity of CP against hydroxyl radical, superoxide anion radical, superoxide anion radical and its reduction force were measured to evaluate their antioxidant activities in vitro. The anti-fatigue effect of CP was evaluated by endurance test (weight-bearing swimming)and biochemical indicators changes in mice. Results:At the concentration of 2.4 mg/mL, the hydroxyl radical, superoxide anion free radical and DPPH free radical scavenging rates of CP were 40.93%, 67.11%, 58.17%, respectively, while the reducing capacity of CP was slightly lower than the same concentration of V_C. Compared with the blank control group, the swimming time of CP-treated mice was prolonged, and the levels of blood urea nitrogen, serum lactic acid, and malondialdehyde were significantly decreased (p<0.05), superoxide dismutase (SOD), hepatic glycogen (HG)and muscle glycogen (MG)levels were significantly increased (p<0.05). Conclusion:CP had strong antioxidant and anti fatigue effect.