Abstract: The optimum technology and fatty acid composition of Batang walnut oil extracted by ultrasonic assisted solvent extraction were studied. The optimum extraction conditions were obtained by single factor and L₉ (3³) orthogonal experiment to study the effects of the ratio of solid to liquid, single ultrasonic time and intermittent time ratio and ultrasonic time on the oil extraction rate. The content of fatty acid, And compared with ultrasonic-assisted enzymatic extraction of walnut oil fatty acid components for comparative analysis. The optimal extraction conditions for ultrasonic extraction of this oil were 1:7.5 g/mL. The ratio of single sonic time to intermittent time was 3:8 s/s, ultrasonic time was 10 min, the yield of oil reached 58.90%. Ultrasound-assisted solvent extraction and ultrasonic-assisted aqueous enzymatic extraction of walnut oil fatty acid Points were linoleic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, linolenic acid, and the relative content difference was not significant (p>0.05).Conclusion:Ultrasound-assisted solvent extraction method is simple, rapid, high yield. Extracted walnut oil is nutritious and of good quality, which provides an accurate and effective reference standard for industrial application, which provides a useful reference for the development and utilization of Batang walnut oil resources.