Abstract: In order to compare the chemical constituents in honey and adulterated honey,the¹H nuclear magnetic resonance(¹H-NMR)and multivariate statistical analysis was used to analyze the chemical constituents of honey and adulterated honey. Twenty four substances were recognized in the¹H-NMR profiling,including seven organic acids,fifteen saccharide and two other substances. It was found that the levels of threonine,proline,turanose,kojibiose,melezitose and phenylalanine were higher,while 2,3-butanediol,acetoin,mannose,5-hydroxymethyl furfural and formic acid were lower in the honey than in adulterated honey. And all these differences were significant(p<0.05).¹H-NMR based metabolomic study on honey could not only be used to identify of adulterated honey,but also analyze the difference components. And it provided the new way and reasonable idea for quality control study of honey.