白花丹参内生真菌HBG16的抗凝血活性研究

张公安; 周晗; 褚翠英; 陈夙; 王玉婷; 贾汝汝; 孙珂; 张芮; 王维维; 张显忠; 张景芳; 李艳玲

doi:10.13386/j.issn1002-0306.2019.08.017

白花丹参内生真菌HBG16的抗凝血活性研究

Anti-coagulation Activity of Endophytic Fungus HBG16 Isolated from Salvia miltiorrhiza Bge.f. alba

摘要

摘要: 目的:考察白花丹参内生真菌菌株HBG16的体外抗凝血活性。方法:采用形态学和分子方法进行属种鉴定;通过PDB培养基对HBG16进行液体发酵,采用甲醇、乙醇、乙酸乙酯和丙酮对菌丝体代谢产物进行提取,考察体外条件下甲醇、乙醇、乙酸乙酯和丙酮四种溶剂粗提物对家兔体外凝血时间(CT)、凝血酶时间(TT)、凝血酶原时间(PT)以及活化部分凝血酶时间(APTT)的影响。结果:白花丹参内生真菌HBG16经鉴定为尖孢镰刀菌;HBG16的菌丝甲醇粗提物对于内源性、外源性凝血都具有一定的延长作用,并且内源性凝血途径最为明显;丙酮、无水乙醇粗提物只作用于内源性凝血途径。结论:白花丹参内生真菌HBG16具有很好的抗凝血作用,可为研制安全无毒的新型抗凝血类药物提供新的资源和方法。

Abstract: Objective:To investigate the anti-coagulation activity of endophytic fungus HBG16 isolated from Salvia miltiorrhiza Bge.f. alba in vitro. Methods:The endophytic fungus HBG16 was identified by the morphological and molecular identification methods and was fermented in PDB culture medium. Four extracts by different solvents(methanol,ethanol,ethyl acetate and acetone)were used to crude extract the mycelium metabolites,and the effects of crude extracts on coagulation time(CT),thrombin time(TT),prothrombin time(PT)and activation of partial thrombin time(APTT)were investigated. Results:Strain HBG16 was identified as Fusarium oxysporum. The methanol crude extract of HBG16 mycelia had extended effect on intrinsic and exogenous coagulation especially on intrinsic coagulation,while acetone and ethanol crude extract only act on intrinsic coagulation. Conclusion:Endophytic fungus HBG16 isolated from Salvia miltiorrhiza Bge.f.alba had significant anti-coagulation activity. It would provid new resources and methods for safe and nontoxic new anticoagulant drugs.

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