Abstract: To analyze the post-harvest quality and shelf life of Agaricus bisporus A15, the effect of relative humidity (RH) of 70%±5%, 80%±5% and 90%±5% under (1±1)℃ on weight loss rate, texture, color, browning index, polyphenol oxidase activity, malondialdehyde content and soluble solids content were detected during 15 days storage. The weight loss rate and TSS content of Agaricus bisporus reached the lowest level at RH 90%±5%, the whiteness decreased by 6.9%, and the hardness remained at a high level. Compared with RH 70%±5%, the PPO activity decreased by 41.5% and the MDA content decreased by 35.9%, both of PPO and MDA were all effectively inhibited at 15 d, indicating that the high humidity surrounding had a certain preservation effect for Agaricus bisporus, and RH 90%±5% was beneficial RH condition to prolong the storage period of Agaricus bisporus.