反枝苋水提物对高脂饮食小鼠的降血脂作用

田程; 王曼力; 杜培革; 安丽萍

doi:10.13386/j.issn1002-0306.2020.19.049

摘要: 目的:研究反枝苋水提物调节高脂血症小鼠血脂的功效。方法:通过高脂肪饮食(high fatdiet,HFD)构建高脂血症小鼠模型,随机分为空白对照组(Con)、高脂模型组(Mod)、洛伐他汀组(Lovastatin,10 mg/kg/d)、反枝苋低剂量组(AR-L,430 mg/kg/d)、反枝苋高剂量组(AR-H,740 mg/kg/d)。空白组给予普通饲料喂养,其余各组给予高脂饲料喂养,同时按相应剂量灌胃给药。干预8周后,检测血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglycerides,TG)、低密度脂蛋白胆固醇(low density lipoprotein Cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)水平,肝脏HE染色观察组织病理学变化。结果:与空白组相比,模型组小鼠肝脏指数以及血清中TC、TG、LDL-C、ALT、AST、MDA水平极显著升高(P<0.01),HDL-C、SOD水平极显著降低(P<0.01);与模型组比较,反枝苋高剂量组小鼠的肝脏指数以及血清中TC、TG、ALT水平极显著降低(P<0.01),SOD、HDL-C水平极显著升高(P<0.01),LDL-C、MDA、AST水平显著降低(P<0.05);与模型比较,反枝苋低剂量组小鼠的肝脏指数以及血清中TC、TG、LDL-C水平显著降低(P<0.05),SOD、HDL-C水平极显著升高(P<0.01),ALT水平极显著降低(P<0.01),MDA、AST水平无显著性差异(P>0.05);HE染色结果显示,反枝苋水提物可明显减轻肝细胞内脂质沉积和肝脏脂肪变性程度。结论:反枝苋水提物能显著降低高脂血症模型小鼠的血脂水平,并能改善其肝功能。

Abstract: Objective:To study the effect of water extract of Amaranthus retroflexus on hyperlipidemia mice. Methods:The mice model of hyperlipidemia was established by high fat diet(HFD). The mice were randomly divided into five groups:Control group(Con),model group(Mod),lovastatin group(Lovastatin,10 mg/kg/d),low dose of water extract of Amaranthus retroflexus group(AR-L,430 mg/kg/d)and high dose of water extract of Amaranthus retroflexus group(AR-H,740 mg/kg/d). The control group was fed with basic feed,the rest groups were fed with high-fat feed,and the drug was given by gavage according to the dosage. After eight weeks,the contents of serum total cholesterol(TC),triglycerides(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),superoxide dismutase(SOD),malondialdehyde(MDA),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)levels in mice were determined.Mice liver histopathology was observed using hematoxylin and eosin(HE)staining.Results:Compared with the control group,the mice liver index extremely significantly increased(P<0.01)in the model group.The levels of mice serum TC,TG,LDL-C,ALT,AST and MDA significantly elevated and HDL-C,SOD extremely significantly reduced(P<0.01). Compared with the model group,the mice liver index extremely significantly increased(P<0.01)in the AR-H group. The levels of mice serum TC,TG and ALT extremely significantly reduced and SOD,HDL-C extremely significantly elevated(P<0.01),and the levels of mice serum MDA,AST significantly reduced(P<0.05). Compared with the model group,the mice liver index significantly increased(P<0.05)in the AR-L group. The levels of mice serum TC,TG and LDL-C significantly reduced(P<0.05),and the levels of mice serum SOD and HDL-C significantly elevated(P<0.01)and ALT extremely significantly reduced(P<0.01),and there were no significant difference in the levels of MDA and AST between the model group and AR-L group(P>0.05).The results of HE staining showed that the water extract of Amaranthus retroflexus could significantly reduce the lipid deposition and fatty degeneration of liver cells. Conclusion:The research shows that the water extract of Amaranthus retroflexus can significantly reduce the blood lipid level of hyperlipidemia model mice and improve their liver function.

反枝苋水提物对高脂饮食小鼠的降血脂作用

Effect of Water Extract of Amaranthus retroflexus on Blood Lipid on Mice Induced by High Fat Diet