Abstract: Objective:To research the effect of delphinidin on glycolysis in breast cancer cell MDA-MB-231.Methods:Cell counting kit-8(CCK8)assay and Ki67 immunohistochemistry were used to test the effect of delphinidin on breast cancer cell MDA-MB-231 proliferation,wound healing assay was used to test the effect of delphinidin on MDA-MB-231cells migration. The effect of delphinidin on glucose uptake,lactate and ATP generation and the activity of hexokinase(HK)were detected in MDA-MB-231cells. Protein expression of pyruvatekinase type M2 pyruvatekinase(PKM2),HK,lactate dehydrogenase-A(LDHA),p-Akt and p-mTOR were detected with western blot(WB). Results:Delphinidin inhibited MDA-MB-231 cells proliferation(Ki67 positive cells)and cell viability. 20 and 40 μmol/L delphinium could reduce the cell survival rate to 75% and 72% respectively(P<0.05). 20 and 40 μmol/L delphinium could reduce the number of Ki67-positive breast cancer MDA-MB-231 cells,the difference was statistically significant(P<0.01). Delphinidin also inhibited glucose uptake,decreased lactate and ATP generation in MDA-MB-231 cells. WB results showed that delphinidin inhibited the protein expression of PKM2,HK,LDHA,p-Akt and p-mTOR. Conclusion:The delphinidin could inhibit glucose uptake and glycolysis in MDA-MB-231 cells. Delphinidin might inhibit MDA-MB-231 cells glycolysis through Akt/mTOR pathway.