Abstract: The hypoglycemic activity of seabuckthorn seed protein peptide in type 2 diabetic mice and the protection effect on kidney were studied. Taking m/m mice as the normal control group(NC)and db/db diabetic mice as the research objects,the positive control group(PC)was administrated gavaged with 300 mg/kg/d metformin,and seabuckthorn seed protein peptide(SSPE)was divided into low,medium and high dose groups(50,150,250 mg/kg/d)for 8 weeks. The body weight,blood glucose,organ index,kidney related indexes and renal histopathological sections were measured to evaluate the hypoglycemic activity and renal protective effect of SSPE. db/db was a kind of diabetic mice with gene deficiency. At the early stage of treatment,the mice in the blank control group(BC)showed symptoms of hyperglycemia,obesity,more drinking,eating and urinating.4 weeks later,the mice in the BC showed more serious symptoms of "three more and less" than those in the SSPE group. After 8 weeks of intervention,the blood glucose value of SSPE group(low,medium and high:19.53,19.94,20.00 mmol/L)were significantly lower than that of BC group(29.56 mmol/L). The organ index and kidney related serum indexes of SSPE group were significantly lower than those of BC group(P<0.05),and the typical symptoms of diabetic nephropathy were also improved. The results showed that SSPE can significantly reduce the blood glucose level of diabetic mice,and exert protective effect on renal function.