Abstract: Objective:To study the effect of Perilla frutescens seed protein on immune system regulation in immunocompromised mice. Methods:Cyclophosphamide(CTX)was injected intraperitoneally to establish a model of immunocompromised mice. The effects of low,medium and high doses of Perilla frutescens seed protein on visceral index,T/B lymphocyte proliferation,serum hemolysin,NK cell killing,phagocytosis index,serum IL-2 and IgG levels in mice were detected. Results:With the increasing of concentration of Perilla frutescens seed protein,the immunomodulatory effect of Perilla frutescens seed protein first increased and then decreased Medium dose of Perilla protein increased T/B lymphocyte proliferation,hemolytic value(HC50),NK cell lethality,macrophage phagocytosis,and serum cytokine IL-2 and IgG levels in immunocompromised mice,and there were significant differences compared with the model group(P<0.01). Conclusion:Perilla frutescens seed protein play a good role in immune regulation in immunocompromised mice.