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中国精品科技期刊2020
汪洪,方昊,冯纬,等. 生晒参-甘草-桂花提取物对Balb/C小鼠运动疲劳抗性的影响[J]. 食品工业科技,2023,44(5):356−362. doi: 10.13386/j.issn1002-0306.2020020240.
引用本文: 汪洪,方昊,冯纬,等. 生晒参-甘草-桂花提取物对Balb/C小鼠运动疲劳抗性的影响[J]. 食品工业科技,2023,44(5):356−362. doi: 10.13386/j.issn1002-0306.2020020240.
WANG Hong, FANG Hao, FENG Wei, et al. Anti-fatigue Effect on Balb/C Mice by Ginseng Glycyrrhiza Root and Osmanthus Flower Extract[J]. Science and Technology of Food Industry, 2023, 44(5): 356−362. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020020240.
Citation: WANG Hong, FANG Hao, FENG Wei, et al. Anti-fatigue Effect on Balb/C Mice by Ginseng Glycyrrhiza Root and Osmanthus Flower Extract[J]. Science and Technology of Food Industry, 2023, 44(5): 356−362. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020020240.

生晒参-甘草-桂花提取物对Balb/C小鼠运动疲劳抗性的影响

Anti-fatigue Effect on Balb/C Mice by Ginseng Glycyrrhiza Root and Osmanthus Flower Extract

  • 摘要: 通过动物实验研究生晒参-甘草-桂花混合提取物对Balb/C小鼠的抗疲劳作用,并初步探讨其抗疲劳机制。本实验将160只Balb/C小鼠随机分为4组:空白组和提取物低、中、高(30、150、300)mg·L−1剂量组。生晒参-甘草-桂花混合后经热水回流工艺提取、过滤和浓缩后,按比例配制为灌胃材料。4组实验小鼠经过环境适应后开始灌胃实验,每日一次,每次2 mL,为期28 d,每周记录各组小鼠体重,并在最后一次灌胃结束后,采用负重游泳实验、常压耐缺氧实验和生化法检测小鼠的肝糖原含量、血尿素氮、血乳酸含量变化及T-SOD活力和MDA含量等指标。结果表明,给药组小鼠游泳前后血乳酸比空白组下降明显;血尿素氮含量增幅与空白组比较明显降低,部分剂量组别具有显著性差异;给药组小鼠血清T-SOD活力(218.71±8.14、217.21±7.82、204.33±7.78 U·mL−1)和肝糖原水平(11.75±1.02、12.03±1.21、11.84±0.67 mg/g)显著高于空白组(121.18±17.58 U·mL−1、7.91±0.89 mg/g);给药组小鼠血清MDA含量(11.24±1.18、13.62±0.65、15.25±0.91 nmol·mL−1)显著低于空白组(21.18±1.62 nmol·mL−1);给药过后可显著延长小鼠负重游泳时间和常压缺氧条件下小鼠存活时间。综上说明生晒参甘草桂花提取物具有明显的抗疲劳作用,其作用机理可能与富含皂苷、黄酮和多糖,调节小鼠肝糖原含量、提高小鼠体内清除氧自由基水平有关。

     

    Abstract: In order to study the anti-fatigue effect of the active ingredients of the ginseng, glycyrrhiza root and osmanthus flower extract on mice, and discuss its anti-fatigue mechanism. The Balb/C mice were randomly divided into 4 groups according to their body weight: Control group and three dose groups (30, 150, 300 mg/L−1). The ginseng glycyrrhiza root and osmanthus flower extract was extracted, filtered and concentrated by hot water reflux process, and then formulated as gavage material in proportion. Gavage saline and low, medium and high doses of extract (once a day) 2 mL each time into the stomach of Balb/C mice that have adapted to the environment. A 28-day gavage trial was performed to record changes in body weight of each group of mice per week. After the last gavage, the hepatic glycogen content, blood urea nitrogen, blood lactate content and T-SOD activity and MDA content were measured by weight-bearing swimming test, normal pressure hypoxia test and biochemical method. The results showed that the serum lactate decreased more significantly after swimming in the administered group of mice than the blank group data, and the increase level of serum urea nitrogen decreased significantly compeared with the blank group, and some dose groups with significant differences. The serum T-SOD levels (218.71±8.14, 217.21±7.82, 204.33±7.78 U·mL−1) and liver glycogen levels (11.75±1.02, 12.03±1.21, 11.84±0.67 mg/g) were significantly higher than those in the blank group (121.18±17.58 U·mL−1, 7.91±0.89 mg/g). And the serum MDA levels of mice in the administered group (11.24±1.18, 13.62±0.65, 15.25±0.91 nmol·mL−1) were significantly lower than those in the blank group (21.18±1.62 nmol·mL−1). The administration of the drug significantly prolonged the survival time of mice under weight-bearing swimming and atmospheric pressure anoxic condition. In conclusion, it shows that ginseng glycyrrhiza root and osmanthus flower extract has significant anti-fatigue effect. The mechanism of action may be related to the rich content of saponins, flavonoids and polysaccharides, which can regulate the liver glycogen content and improve the level of oxygen free radical scavenging in mice.

     

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