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中国精品科技期刊2020
屈云, 贺苏皖, 赵燕英, 施春雷, 陈娟, 于基成, 唐俊妮. 成都市生鲜猪肉MRSA分离株的流行特征分析[J]. 食品工业科技, 2021, 42(3): 82-89. DOI: 10.13386/j.issn1002-0306.2020040085
引用本文: 屈云, 贺苏皖, 赵燕英, 施春雷, 陈娟, 于基成, 唐俊妮. 成都市生鲜猪肉MRSA分离株的流行特征分析[J]. 食品工业科技, 2021, 42(3): 82-89. DOI: 10.13386/j.issn1002-0306.2020040085
QU Yun, HE Suwan, ZHAO Yanying, SHI Chunlei, CHEN Juan, YU Jicheng, TANG Junni. Epidemiological Characteristics of MRSA Strains Isolated from Fresh Pork Sources of Chengdu City[J]. Science and Technology of Food Industry, 2021, 42(3): 82-89. DOI: 10.13386/j.issn1002-0306.2020040085
Citation: QU Yun, HE Suwan, ZHAO Yanying, SHI Chunlei, CHEN Juan, YU Jicheng, TANG Junni. Epidemiological Characteristics of MRSA Strains Isolated from Fresh Pork Sources of Chengdu City[J]. Science and Technology of Food Industry, 2021, 42(3): 82-89. DOI: 10.13386/j.issn1002-0306.2020040085

成都市生鲜猪肉MRSA分离株的流行特征分析

Epidemiological Characteristics of MRSA Strains Isolated from Fresh Pork Sources of Chengdu City

  • 摘要: 目的:分析成都市各地区生鲜猪肉样品中耐甲氧西林金黄色葡萄球菌(Methicillin-resistant Staphylococcus aureus,MRSA)的流行特征。方法:对鉴定出的MRSA菌株进行SCCmec分型、spa分型以及多位点序列(MLST)分型分析,同时运用PCR技术对MRSA菌株携带的多种毒力基因、生物被膜形成相关基因、耐消毒剂基因以及耐药基因进行检测,并采用K-B纸片扩散法调查MRSA菌株的耐药表型。结果:本研究从297株猪肉源金黄色葡萄球菌中筛选出24株MRSA菌株,分离率为8.08%。MRSA菌株主要以ST88-IVa-t1376、ST59-IVa-t437和ST9-IVb-t3433型为主,携带耐药基因3~7个;所有菌株均表现出对氨苄西林和青霉素G耐药,并且存在多重耐药情况;分离菌株携带多种肠毒素基因,其中MRSA8和MRSA13菌株携带了完整的egc基因簇;MRSA分离菌株均携带clfB、eno、icaBC和sasG等生物被膜形成相关基因;携带耐消毒剂基因(qacA/B、qacC和qacG)的菌株占比为62.5%,其中以qacG为主要携带基因类型。另外,79.17%的MRSA菌株携带溶血素基因(hla、hlb),所有菌株中杀白细胞素基因(pvl)均未检出。结论:成都市猪肉源MRSA的流行可能存在一定的交叉污染,应加强动物源食品携带MRSA菌株的监测。

     

    Abstract: Objective:The epidemiological characteristics of methicillin-resistant Staphylococcus aureus(MRSA)isolated from fresh pork samples were investigated.Methods:MRSA isolates from fresh pork samples were conducted for MLST,spa and SCCmec typing. The enterotoxin genes,disinfectant resistance gene,biofilm genes and drug resistance genes carried by MRSA isolates were detected by polymerase chain reaction(PCR)method. The antibiotics sensitivity of MRSA was also investigated by K-B disc diffusion method. Results:In this study,24 strains of MRSA were screened from 297 S. aureus isolates of fresh pork origin,with an isolation rate of 8.08%.The main types of MRSA isolates were ST88-IVa-t1376,ST59-IVa-t437 and ST9-IVb-t3433. Most isolates carried 3~7 antibiotics resistant tested genes. All the MRSA strains were resistant to ampicillin and penicillin G,and multi-drug resistances existed. At the same time,MRSA strains carried multiple enterotoxin genes,of which,MRSA8 and MRSA13 carried the complete egc gene cluster. These isolates contained biofilm-forming related genes clfB,eno,icaBC and sasG. The anti-disinfectant genes qacA/B,qacC and qacG were carried by 62.5% of the isolates,and qacG gene was the main type. In addition,79.17% of MRSA carried hemolysin genes(hla,hlb). The pvl gene was not detected in all MRSA strains.Conclusion:The prevalence of pork-sourced MRSA in Chengdu might have cross-contamination and the monitoring of MRSA strains carried by animal-derived foods should be strengthened.

     

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