Abstract: After atmospheric and room temperature plasma(ARTP)and ultraviolet(UV)compound mutation,a high-performance strains of Metarhizium Anisopliae was selected. By comparing the speed of sporulation and the size of growth diameter,selected with the ability of anti-ultraviolet and virulence as the re-screening index,and tested for heat resistance and genetic stability,a good strain AU34 with high sporulation,UV tolerance,strong virulence and stable genetic was selected at the time of ARTP 40 s and UV 120 s. The sporulation time of the strain was faster than the original strain,and the sporulation amount was 1.63±0.22(10⁸ cell/cm²),which was 63.81% higher than the original strain. After 5 min of UV irradiation,the survival rate of AU34 was 3.18%,which was more resistant to UV irradiation than the original strain. The toxicity of AU34 to Plutella xylostella L. was high. The adjusted mortality rate was 85.71% and LT₅₀ was 6.12 days. Compared with the original strain,the heat resistance of AU34 was also improved. After subculture for 6 generations,the sporulation of strain AU34 had no obvious change and had good genetic stability.