• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
何策,王超,陈纯,等. 桑树桑黄总三萜提取工艺优化及其降血脂、抗氧化活性研究[J]. 食品工业科技,2021,42(7):208−215. doi: 10.13386/j.issn1002-0306.2020060237.
引用本文: 何策,王超,陈纯,等. 桑树桑黄总三萜提取工艺优化及其降血脂、抗氧化活性研究[J]. 食品工业科技,2021,42(7):208−215. doi: 10.13386/j.issn1002-0306.2020060237.
HE Ce, WANG Chao, CHEN Chun, et al. Optimization of Extraction Technology of Total Triterpenoids from Inonotus sanghuang and Their Hypolipidemic and Antioxidant Activities[J]. Science and Technology of Food Industry, 2021, 42(7): 208−215. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060237.
Citation: HE Ce, WANG Chao, CHEN Chun, et al. Optimization of Extraction Technology of Total Triterpenoids from Inonotus sanghuang and Their Hypolipidemic and Antioxidant Activities[J]. Science and Technology of Food Industry, 2021, 42(7): 208−215. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060237.

桑树桑黄总三萜提取工艺优化及其降血脂、抗氧化活性研究

Optimization of Extraction Technology of Total Triterpenoids from Inonotus sanghuang and Their Hypolipidemic and Antioxidant Activities

  • 摘要: 选用超声波法提取桑树桑黄子实体中的三萜类化合物,在单因素试验的基础上采用响应面法优选最佳方案,并对其体外降血脂和抗氧化活性进行了测定。结果表明,超声波法提取桑树桑黄总三萜的最优条件为:提取时间49 min、乙醇浓度为80%、提取温度为61 ℃,料液比为1:22(g/mL),总三萜得率可达12.32% ± 0.17%。质量浓度为80 mg/mL的总三萜体外结合甘氨胆酸钠、牛磺胆酸钠、胆酸钠的能力为同剂量考来烯胺的50.93%、52.14%和43.06%,说明其具有较好的体外降血脂活性。总三萜具有较好的还原能力,并能有效清除DPPH和ABTS自由基,其IC50值分别为0.304和0.520 mg/mL,表明其具有较好的体外抗氧化活性。

     

    Abstract: The ultrasonic-assisted extraction process of total triterpenoids from Inonotus sanghuang was employed in this paper. Based on the single factor experiments, the response surface methodology (RSM) was used to determine the optimal extraction condition, and its hypolipidemic and antioxidant activities were primarily evaluated. The results of optimum extraction conditions were as follow: Extraction time 49 min, ethanol concentration 80%, ultrasonic temperature 61 ℃, ratio of solid to liquid 1:22 (g/mL). Under these conditions, the yield of total triterpenoids was 12.32% ± 0.17%. The binding ability of 80 mg/mL triterpenoids to sodium glycylcholate, sodium taurocholate and sodium cholate in vitro was 50.93%, 52.14% and 43.06% of that of colenamine in the same dose, which indicated that the total triterpenoids of Inonotus sanghuang had the activity of lowering blood lipids in vitro. The iron ions reduction results showed that the total triterpenoids had certain reducing power. The IC50 values of scavenging capacities of DPPH and ABTS free radical was 0.304 and 0.520 mg/mL, respectively, which showed it possessed good antioxidants in vitro.

     

/

返回文章
返回