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中国精品科技期刊2020
张维捷,谢兴非,闫鹤. 一种复方中草药对阿尔兹海默症模型小鼠认知功能障碍及肠道菌群的影响[J]. 食品工业科技,2021,42(13):345−350. doi: 10.13386/j.issn1002-0306.2020080074.
引用本文: 张维捷,谢兴非,闫鹤. 一种复方中草药对阿尔兹海默症模型小鼠认知功能障碍及肠道菌群的影响[J]. 食品工业科技,2021,42(13):345−350. doi: 10.13386/j.issn1002-0306.2020080074.
ZHANG Weijie, XIE Xingfei, YAN He. Effect of a Compound Chinese Herbal Medicine on Cognitive Dysfunction and Gut Microbiota in the Model Mouse with Alzheimer’s Disease[J]. Science and Technology of Food Industry, 2021, 42(13): 345−350. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080074.
Citation: ZHANG Weijie, XIE Xingfei, YAN He. Effect of a Compound Chinese Herbal Medicine on Cognitive Dysfunction and Gut Microbiota in the Model Mouse with Alzheimer’s Disease[J]. Science and Technology of Food Industry, 2021, 42(13): 345−350. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080074.

一种复方中草药对阿尔兹海默症模型小鼠认知功能障碍及肠道菌群的影响

Effect of a Compound Chinese Herbal Medicine on Cognitive Dysfunction and Gut Microbiota in the Model Mouse with Alzheimer’s Disease

  • 摘要: 选用阿尔兹海默症转基因小鼠模型,探究以逍遥散为基础的复方中草药改善阿尔兹海默症(Alzheimer's disease, AD)症状的潜在作用机制。以行为学(Morris水迷宫实验逃避潜伏期、穿越目标平台次数)及16S rRNA基因测序结果为参考指标,探究复方中草药对AD模型小鼠认知功能障碍和粪便菌群结构、组成及功能的影响。结果显示,与AD组相比,复方中草药(Compound Chinese Herbal Medicine, CCHM)组逃避潜伏期显著缩短,穿越平台次数显著增加(P<0.05),一定程度上提高了小鼠的认知功能;另外与AD组相比,CCHM提高了粪便菌群的Alpha多样性,调整菌群结构显著偏离AD组,并接近于野生型(wild-type, WT)正常小鼠。进一步分析发现,与WT组相比,OTU345、OTU380和OTU264的相对丰度在AD组显著降低(P<0.05),而CCHM干预后显著提高(P<0.05),其中OTU345、OTU380注释到可产生丁酸盐的毛螺菌科(Lachnospiraceae)。此外,通过对小鼠肠道菌群科水平进行分析,同样发现相比于WT组,Lachnospiraceae相对丰度在AD组中显著降低(P<0.05),而复方中草药干预后丰度显著增加(P<0.05)。最后由16S rRNA基因功能预测结果显示,CCHM对于AD模型小鼠肠道微生物功能紊乱有一定的调节作用。因此,CCHM可改善AD小鼠相关症状,且影响了其肠道菌群,为明确其潜在作用机制提供了理论依据。

     

    Abstract: In this study, the model of transgenic mouse with Alzheimer’s disease (AD) was used to explore the potential mechanism of Compound Chinese Herbal Medicine (CCHM) based on Xiaoyaosan in alleviating the symptoms of AD. The effects of CCHM on cognitive dysfunction and fecal microbiota structure, composition and function in AD model mouse was investigated by using behavioral tests (including escaped latency and number of crossing the target platformin Morris water maze experiment) and 16S rRNA gene sequencing. Compared with AD group, the behavioral tests results showed that CCHM could improve the cognitive function of mouse, including shorten escaped latency and increased the number of crossing the platform (P<0.05). After CCHM treatment, the Alpha diversity of fecal microbiota increased, and the fecal bacterial profiles of the CCHM group deviated from those of the AD group, and the microbial communities of the CCHM group were close to those of the wild-type (WT) normal mouse. Furthermore, compared with WT group, the relative abundance of OTU345, OTU380 and OTU264 significantly decreased in AD group (P<0.05), while it was obviously enhanced after CCHM intervention (P<0.05), in which OTU345 and OTU380 belonged to Lachnospiraceae, a butyrate-producing bacterium. In addition, by analyzing the level of intestinal microflora in mice, it was also found that, compared with WT group, the relative abundance of Lachnospiraceae in AD group decreased significantly (P<0.05), while the relative abundance of Lachnospiraceae increased significantly after CCHM intervention (P<0.05).Finally, the 16S rRNA gene function prediction results showed that CCHM had a certain regulatory effect on gut microbial dysfunction in AD model mice. Therefore, CCHM could improve the symptoms of AD mice and affected their intestinal flora, providing a theoretical basis for the identification of its potential mechanism of action.

     

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