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中国精品科技期刊2020
程晓晓,张邈馨,王嫚,等. 苦瓜桑叶复合颗粒对高脂饮食诱导肥胖大鼠脂糖代谢及肠粘膜屏障异常的改善作用[J]. 食品工业科技,2021,42(15):337−342. doi: 10.13386/j.issn1002-0306.2020110096.
引用本文: 程晓晓,张邈馨,王嫚,等. 苦瓜桑叶复合颗粒对高脂饮食诱导肥胖大鼠脂糖代谢及肠粘膜屏障异常的改善作用[J]. 食品工业科技,2021,42(15):337−342. doi: 10.13386/j.issn1002-0306.2020110096.
CHENG Xiaoxiao, ZHANG Miaoxin, WANG Man, et al. Improving Effect of the Composite Granules of Bitter Melon and Mulberry Leaf on Lipid-Glucose Metabolism and Abnormal Intestinal Mucosal Barrier in High-Fat Diet-Induced Obese Rats[J]. Science and Technology of Food Industry, 2021, 42(15): 337−342. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110096.
Citation: CHENG Xiaoxiao, ZHANG Miaoxin, WANG Man, et al. Improving Effect of the Composite Granules of Bitter Melon and Mulberry Leaf on Lipid-Glucose Metabolism and Abnormal Intestinal Mucosal Barrier in High-Fat Diet-Induced Obese Rats[J]. Science and Technology of Food Industry, 2021, 42(15): 337−342. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110096.

苦瓜桑叶复合颗粒对高脂饮食诱导肥胖大鼠脂糖代谢及肠粘膜屏障异常的改善作用

Improving Effect of the Composite Granules of Bitter Melon and Mulberry Leaf on Lipid-Glucose Metabolism and Abnormal Intestinal Mucosal Barrier in High-Fat Diet-Induced Obese Rats

  • 摘要: 目的:研究苦瓜桑叶复合颗粒对高脂饮食诱导肥胖大鼠脂糖代谢及肠粘膜屏障异常的改善作用,为苦瓜桑叶复合颗粒的进一步开发应用提供数据支持。方法:SD大鼠随机分为空白对照组、高脂肥胖模型组、苦瓜桑叶复合颗粒低剂量组和高剂量组。除空白对照组外,其他组大鼠均给予高脂饲料建立肥胖模型。苦瓜桑叶复合颗粒低、高剂量组每天分别灌胃0.5和1 g/kg的苦瓜桑叶复合颗粒,其他组以相同方法灌胃等体积生理盐水,连续给药8周。测定大鼠体重及血糖血脂水平,包括葡萄糖(glucose,GLU)、糖化血清蛋白(glycated serum proteins,GSP)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipid-cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipid-cholesterol,LDL-C)等。通过苏木精-伊红染色观察大鼠肝脏和结肠组织形态变化,并检测结肠紧密连接蛋白Occludin的表达。结果:苦瓜桑叶复合颗粒可有效调控高脂饮食诱导肥胖大鼠的体重和血糖血脂水平,使其恢复正常。病理切片显示苦瓜桑叶复合颗粒可降低肥胖大鼠肝脏脂滴的形成并改善结肠粘膜腺体异常形态。同时,苦瓜桑叶复合颗粒可有效提升肥胖大鼠结肠Occludin蛋白的表达(P<0.05),改善肠粘膜紧密连接状态。结论:苦瓜桑叶复合颗粒对高脂饮食诱导的肥胖大鼠脂糖代谢异常具有明显改善作用,并可一定程度修复肥胖引起的肠道粘膜损伤,此研究对苦瓜桑叶复合颗粒的开发及相关机制的阐明具有重要意义。

     

    Abstract: Objective: To study the effect of the composite granules of bitter melon and mulberry leaf (CGBM) on improving dysfunction of obesity rats induced by high-fat diet. An explorative study was conducted on CGBM for its regulation of lipid-glucose metabolism and intestinal mucosal barrier in high-fat diet-induced obese rats, in order to provide data support for the further development of CGBM. Methods: SD rats were randomly divided into control group, model group, CGBM low-dose and high-dose group. The rats in the control group were given basic diet, whereas the others were fed in the high-fat diet to establish obesity model. The rats in the low-dose (0.5 g/kg) and high-dose (1.0 g/kg) groups were given CGBM by intragastric administration per day for 8 weeks. The rats in other groups were given the same volumes of normal saline by the same method for 8 weeks. The body weight, serum glucose and lipid levels (such as GLU, GSP, TC, TG, HDL-C, LDL-C) of rats were measured. The morphology of liver and colon tissues were observed by HE staining, and the expression of tight junction protein occludin in colon tissues were detected. Results: CGBM could effectively reduce the body weight, and lower the high level of serum glucose and lipid in obese rats. The phenomenon of excessive lipid droplet in liver and the abnormal morphology of glands in colon mucosa were also improved by CGBM. The results also indicated that the CGBM could effectively ameliorate the lower expression level of occludin protein in obesity rats (P<0.05). Conclusion: CGBM has the obvious improvement effect on dysfunction of glucose and lipid metabolism in obesity rats induced by high-fat diet. The hepatic and intestinal injuries caused by high-fat diet were also repaired by CGBM. This study would have an important significance on accelerating the development of CGBM and its mechanism research.

     

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