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中国精品科技期刊2020
刘大伟. 黄芩多糖大孔树脂纯化工艺优化及其抑菌活性[J]. 食品工业科技,2021,42(15):183−188. doi: 10.13386/j.issn1002-0306.2020110170.
引用本文: 刘大伟. 黄芩多糖大孔树脂纯化工艺优化及其抑菌活性[J]. 食品工业科技,2021,42(15):183−188. doi: 10.13386/j.issn1002-0306.2020110170.
LIU Dawei. Optimization Purification Process of Polysaccharides from Scutellaria baicalensis Georgi by Macroporous Resins and Its Antibacterial Activity[J]. Science and Technology of Food Industry, 2021, 42(15): 183−188. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110170.
Citation: LIU Dawei. Optimization Purification Process of Polysaccharides from Scutellaria baicalensis Georgi by Macroporous Resins and Its Antibacterial Activity[J]. Science and Technology of Food Industry, 2021, 42(15): 183−188. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110170.

黄芩多糖大孔树脂纯化工艺优化及其抑菌活性

Optimization Purification Process of Polysaccharides from Scutellaria baicalensis Georgi by Macroporous Resins and Its Antibacterial Activity

  • 摘要: 为优化大孔树脂纯化黄芩粗多糖的工艺,比较不同类型树脂的静态吸附与解吸性能,确定适宜树脂型号,研究其吸附机理。通过动态吸附与洗脱试验确定最佳纯化工艺,同时利用滤纸片法考察黄芩多糖对大肠杆菌、枯草杆菌和金黄色葡萄球菌的抑菌效果。结果表明,AB-8型大孔树脂对黄芩多糖的吸附过程符合Langmuir模型特征,最佳纯化工艺条件为:3 mg/mL的黄芩粗多糖溶液(pH 5.0) 60 mL,以2.0 mL/min流速上样至AB-8型树脂饱和吸附后,采用75%乙醇溶液,以1.0 mL/min流速洗脱,纯化后的多糖含量从26.25%提高至77.12%。黄芩多糖对三种供试菌具有不同程度的抑制作用,其中对大肠杆菌的抑制活性最强,对大肠杆菌、金黄色葡萄球菌及枯草杆菌的最小抑菌浓度分别为0.625、0.625、1.25 mg/mL。因此该纯化工艺效率较高,纯化后的黄芩多糖具有较好的抑菌活性,可为相关资源的开发提供参考。

     

    Abstract: To optimize the purification conditions of polysaccharides extract from Scutellaria baicalensis Georgi by macroporous resin, the suitable macroporous resin was selected through performance comparison of static adsorption and elution of macroporous resins of different types, and the optimum conditions were confirmed by dynamic adsorption and elution tests. Meanwhile, the resistant abilities against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis were detected by filtering paper method. The results showed that the adsorption curves that AB-8 macroporous resin adsorbed polysaccharides of Scutellaria baicalensis Georgi accorded with the Langmuir mode feature. The optimum purification conditions were as follows: 60 mL polysaccharides extract solution of concentration 3 mg/mL (pH 5.0) was made and adsorbed by AB-8 macroporous resin with loading flow rate 2.0 mL/min, and then eluted by 75% ethanol with elution flow rate 1.0 mL/min. Under these conditions, the total polysaccharides content increased from 26.25% to 77.12%. The polysaccharides of Scutellaria baicalensis Georgi had inhibitory effects varying degrees on the four tested bacteria and the strongest inhibitory activity was resistant against Escherichia coli. The minimum inhibitory concentrations of Escherichia coli, Staphylococcus aureus, and Bacillus subtilis were 0.625, 0.625 and 1.25 mg/mL, respectively. This process had better purified efficiency, and polysaccharides after purification had better antibacterial activities, therefore it provides a reference for corresponding resource development.

     

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