Abstract:
With the amniotic bacteria sub-entity as the main raw material, by cellulase, papaya protease complex enzyme solution, ultrasound assisted extraction to obtain
Morchella esculenta polysaccharides
, further through the addition of auxiliary ingredients to develop amniotic bacteria polysaccharide tablets. Single-factor and response surface experimental optimization obtained the best conditions for the optimal extraction of
Morchella esculenta polysaccharides for liquid ratio of 29:1 mL/g, enzymatic pH5.1, enzymatic time 2 h, enzyme dosage 1.4%, ultrasonic time 20 min, the rate of extraction reached up to 9.21%; Single-factor experiments and orthogonal experiments determined that the best type of filler for polysaccharide tablets of amniotic bacteria was glycol:sorbitol: 1:1, the composition of the ingredients as fillers and polysaccharide leaching paste ratio was 8:1, vitamin C was added 5 mg/g, the addition of stingric acid magnesium was 0.8%, and the comprehensive score of the tablets was 86.4 under this condition. When the tablet concentration was 10 mg/mL, the antioxidant activity results were: hydroxy radical clearance of 46.78% and DPPH radical clearance of 75.38%, with good antioxidant activity.
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