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中国精品科技期刊2020
夏蕴实,孙印石,刘畅,等. 三种动物油脂对大鼠急性胃粘膜损伤的影响[J]. 食品工业科技,2021,42(19):369−375. doi: 10.13386/j.issn1002-0306.2020110238.
引用本文: 夏蕴实,孙印石,刘畅,等. 三种动物油脂对大鼠急性胃粘膜损伤的影响[J]. 食品工业科技,2021,42(19):369−375. doi: 10.13386/j.issn1002-0306.2020110238.
XIA Yunshi, SUN Yinshi, LIU Chang, et al. Effects of Three Animal Oils on Acute Gastric Mucosal Injury in Rats[J]. Science and Technology of Food Industry, 2021, 42(19): 369−375. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110238.
Citation: XIA Yunshi, SUN Yinshi, LIU Chang, et al. Effects of Three Animal Oils on Acute Gastric Mucosal Injury in Rats[J]. Science and Technology of Food Industry, 2021, 42(19): 369−375. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110238.

三种动物油脂对大鼠急性胃粘膜损伤的影响

Effects of Three Animal Oils on Acute Gastric Mucosal Injury in Rats

  • 摘要: 目的:研究三种动物油脂对乙醇致大鼠胃粘膜损伤的影响。方法:分别以低(500 mg/kg)、高剂量(850 mg/kg)的鹿油、牛油、猪油对大鼠连续灌胃30 d后,除正常组外,其余各组大鼠灌胃给予无水乙醇1.0 mL/只,1 h后麻醉,心脏取血,取胃组织并称重,对大鼠胃粘膜进行形态学观察、组织病理学检查以及急性损伤指标的测定,并检测各组大鼠血清中超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)含量,利用ELISA法测定血清中肿瘤坏死因子α(TNF-α)、白介素-6(IL-6)的含量变化,并用RT-PCR技术检测促红细胞生成素(EPO)和促红细胞生成素受体(EPOR)的mRNA表达水平。结果:与模型组相比,各给药组大鼠在各时期体重均无明显变化,鹿油预处理组大鼠胃重量/体质量极显著降低(P<0.001),胃粘膜表面较少出血带且出血带较细,细胞排列紧密有序,炎性浸润减少,胃粘膜充血面积、损伤积分指数均极显著降低(P<0.001),能极显著提高GSH-Px活力、SOD活性(P<0.01)并显著降低MDA水平(P<0.05),进而提高机体抗氧化能力来保护胃黏膜,鹿油高度显著降低血清中炎症因子TNF-α和IL-6水平,能够调控乙醇对胃粘膜造成损伤所产生的炎症因子,使EPO和EPOR的mRNA表达降低;牛油预处理组大鼠胃粘膜表面多条细长出血带,颜色略深,细胞排列较为有序,胃粘膜充血面积、损伤积分指数降低,可极显著提高SOD活力(P<0.001)、明显降低MDA水平来提升抗氧化能力保护胃黏膜;猪油预处理组大鼠胃粘膜表面有多条较宽出血带,颜色较深,对急性胃粘膜损伤无明显影响。结论:三种动物油脂对急性胃粘膜损伤的保护作用依次是:鹿油>牛油>猪油,鹿油对大鼠急性胃粘膜损伤具有明显保护作用,牛油保护作用较弱,猪油则无明显保护作用。

     

    Abstract: Objective: To study the effects of three kinds of animal fats on gastric mucosa damage induced by ethanol in rats. Methods: Low (500 mg/kg) and high doses (850 mg/kg) of deer oil, butter and lard were given to rats by gavage for 30 days. Except for the normal group, rats in the other groups were given 1.0 mL of absolute ethanol per rat. One hour later, blood was taken from anesthesia, and gastric tissue was taken. Morphological observation, histopathological examination and the determination of acute injury indexes were performed on the gastric mucosa of rats. Detect the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in serum of rats in each group. The ELISA method was used to determine the serum levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and RT-PCR was used to detect erythropoietin (EPO) and erythropoietin receptor (EPOR) mRNA expression level. Results: Compared with the model group, the weight of rats in each administration group did not change significantly in each period. The stomach weight/body mass of the rats in the deer oil pretreatment group was extremely significantly reduced(P<0.001), and the gastric mucosal surface had fewer bleeding bands and thinner bleeding bands. The cells were arranged tightly and orderly, the inflammatory infiltration was reduced, the gastric mucosal congestion area and the injury score index were extremely significantly redu(P<0.001)ced, which could significantly increase the activity of GSH-Px, SOD activity(P<0.01) and significantly reduced the level of MDA(P<0.05), thereby improving the body's antioxidant capacity to protect the stomach. In mucosa, deer oil highly significantly reduced the levels of inflammatory factors TNF-α and IL-6 in the serum, could regulate the inflammatory factors produced by the damage of ethanol to the gastric mucosa, and reduced the mRNA expression of EPO and EPOR; rats in the butter pretreatment group, there were many slender bleeding bands on the surface of the gastric mucosa, the color was slightly darker, the cell arrangement was more orderly, the gastric mucosal congestion area and the injury score index were reduced, which could significantly increase the SOD activity(P<0.001) and reduce the MDA level to enhance the antioxidant capacity and protected the gastric mucosa; In the lard pretreatment group, there were many wide bleeding bands on the gastric mucosa surface, which were darker in color, and had no obvious effect on acute gastric mucosal injury. Conclusion: The protective effects of three animal fats on acute gastric mucosal injury are in order: deer oil>butter>lard. Deer oil has a significant protective effect on acute gastric mucosal injury in rats. The protective effect of butter is weak, while lard has no obvious protective effect.

     

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