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中国精品科技期刊2020
侯粲,肖杰,王黎明,等. 六堡茶改善痰湿质功效评价及基于肠道菌群调节的祛湿机制研究[J]. 食品工业科技,2021,42(21):361−369. doi: 10.13386/j.issn1002-0306.2020120100.
引用本文: 侯粲,肖杰,王黎明,等. 六堡茶改善痰湿质功效评价及基于肠道菌群调节的祛湿机制研究[J]. 食品工业科技,2021,42(21):361−369. doi: 10.13386/j.issn1002-0306.2020120100.
HOU Can, XIAO Jie, WANG Liming, et al. Effect Evaluation of Liupao Tea on Improving Phlegm-dampness Constitution and Mechanism of Clearing Damp on Intestinal Microbiota[J]. Science and Technology of Food Industry, 2021, 42(21): 361−369. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120100.
Citation: HOU Can, XIAO Jie, WANG Liming, et al. Effect Evaluation of Liupao Tea on Improving Phlegm-dampness Constitution and Mechanism of Clearing Damp on Intestinal Microbiota[J]. Science and Technology of Food Industry, 2021, 42(21): 361−369. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120100.

六堡茶改善痰湿质功效评价及基于肠道菌群调节的祛湿机制研究

Effect Evaluation of Liupao Tea on Improving Phlegm-dampness Constitution and Mechanism of Clearing Damp on Intestinal Microbiota

  • 摘要: 为研究六堡茶调理亚健康状态痰湿体质的有效性,并探究其作用机制,本研究以六堡茶新茶和五年陈化茶(10 g,200 mL沸水浸泡2 min口服,每日2次)作为干预手段,纳入亚健康状态痰湿质人群进行随机、对照的临床试验,进行90 d的标准化干预,记录干预前后与痰湿质有关的中医诊断指标、血液生化指标,并重点分析肠道菌群的变化。结果显示,六堡茶新茶干预组和陈茶干预组在干预后痰湿质转化分均有下降趋势,其中六堡茶新茶干预组在干预后的25、45和90 d三个时间点痰湿质转化分差异有显著性(P<0.05)。六堡茶新茶及五年陈茶干预均有助于痰湿质体质改善,其中新茶起效较快。生化结果显示,六堡茶陈茶和新茶均具有降低胆红素的作用,陈茶作用较为明显,差异具有统计学意义(P<0.05)。肠道菌群分析显示,六堡茶干预后,六堡茶新茶干预组拟杆菌门(Bacterioidetes)水平下降为0.49±0.17、厚壁菌门(Firmicutes)水平上升至0.42±0.23,六堡茶陈茶干预组拟杆菌门(Bacterioidetes)水平下降为0.48±0.35、厚壁菌门(Firmicutes)水平上升至0.45±0.08;嗜胆菌(Bilophila)的相对丰度提高至六堡茶新茶干预组的0.0024±0.0013和六堡茶陈茶干预组的0.0022±0.0034,改变了肠道菌群中的厚壁菌门和拟杆菌门的相对比例, 促进嗜胆菌增殖。与代谢疾病、能量代谢有关的萨特氏菌属(Sutterella)、Izimaplasmatales菌和瘤胃球菌属(Ruminococcus)的相对丰度水平发生改变,因此六堡茶可能通过调节肠道微生态,改善痰湿质等亚健康人群的代谢状态。

     

    Abstract: To investigate the efficacy of Liupao tea on regulating phlegm-dampness constitution in sub-health state and the mechanism, a randomized, controlled clinical trial of 90-day was taken using Liupao fresh and aged tea with the intervention dose of 10 g soaked in boiling water of 200 ml for 2 min, twice a day as the intervention means. Traditional Chinese medicine diagnostic indexes and blood biochemical indexes related to phlegm-dampness were recorded before and after intervention, and the change of intestinal flora was analyzed. The results indicated that the phlegm-dampness quality translation score showed a decreasing trend in the middle and end of the intervention among the fresh tea intervention group and aged tea intervention group. The ANOVA results showed the phlegm-dampness quality translation score of fresh tea intervention group had significant differences at three time points of 25, 45 and 90 d after intervention (P<0.05). The intervention of Liupao tea could improve the phlegm-dampness constitution slowly and the effect of fresh tea was slightly faster. Biochemical results showed that Liupao tea had the effect of reducing bilirubin (total bilirubin and direct bilirubin). The effect of aged tea was obvious and the difference was statistically significant (P<0.05). Intestinal flora results showed that the level of Bacterioidetes decreased to 0.49±0.17 in fresh tea intervention group and 0.48±0.35 in aged tea intervention group, while the level of Firmicutes increased to 0.42±0.23 in fresh tea intervention group and 0.45±0.08 in aged tea intervention group after intervention. The relative abundance of Bilophila increased to 0.0024±0.0013 in fresh tea intervention group and 0.0022±0.0034 in aged tea intervention group. Liupao tea changed the relative proportion of Firmicutes and Bacteroidetes in the intestinal flora, promoted the increase of Bilophila, thus Liupao tea may improve the metabolism of blood bilirubin by regulating the bile acid pathway. The relative abundance of Sutterella, Izimaplasmatales and Ruminococcus, which are related to metabolic diseases and energy metabolism, were changed during the experiment. Therefore, Liupao tea may improve the metabolic state of sub-health people by regulating intestinal microbiota.

     

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