Abstract: Lycium barbarum flavone is the main effective component of Chinese medicinal material Lycium barbarum, and it is also the main evaluation index of its quality. This paper investigated the effects of ethanol reflux extraction, high-speed shear-assisted ethanol extraction, high-speed shear-assisted DESs extraction, and ultrasound-assisted DESs extraction on the extraction amount of Lycium barbarum flavonoids. The best extraction method was selected, and the best extraction conditions were obtained by single factor experiments and orthogonal experiment. Based on the best extraction method, SIMCA 14.1 was used. The software analyzed the correlation between the ecological factors in different producing areas and the content of Lycium barbarum flavonoids. The results showed that the extraction efficiency of Lycium barbarum flavonoids with high-speed shearing assisted DESs was the best, the optimal extraction process was as follows: Speed 14000 r/min, material-to-liquid ratio 1:20 g/mL, extraction time 5 min, the average flavonoid extraction volume was 7.11 mg/g. The flavonoid content of Lycium barbarum from different origins in descending order was: Ningxia Yinchuan>Ningxia Zhongwei>Gansu Guazhou>Ningxia Guyuan>Xinjiang Jinghe>Ningxia Zhongning>Gansu Baiyin>Gansu Wuwei>Gansu Yumen>Xinjiang Altai>Xinjiang Yining>Qinghai Dulan>Qinghai Golmud>South Korea b>South Korea a. SIMCA 14.1 Software analysis showed that the positive regression coefficient value of Lycium barbarum flavonoids content and temperature and frost-free period was the largest, and the absolute value of negative regression coefficient with altitude was the largest. The most important ecological factors affecting the content of Lycium barbarum flavonoids were temperature and frost-free period.