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中国精品科技期刊2020
李慧,潘思轶,徐晓云. 芹菜素黄酮氧苷、黄酮碳苷同分异构体抗炎活性评价[J]. 食品工业科技,2022,43(13):345−352. doi: 10.13386/j.issn1002-0306.2021100016.
引用本文: 李慧,潘思轶,徐晓云. 芹菜素黄酮氧苷、黄酮碳苷同分异构体抗炎活性评价[J]. 食品工业科技,2022,43(13):345−352. doi: 10.13386/j.issn1002-0306.2021100016.
LI Hui, PAN Siyi, XU Xiaoyun. Anti-inflammation Activity Evaluation of Apigenin-O-Glycosides and Apigenin-C-Glycosides[J]. Science and Technology of Food Industry, 2022, 43(13): 345−352. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100016.
Citation: LI Hui, PAN Siyi, XU Xiaoyun. Anti-inflammation Activity Evaluation of Apigenin-O-Glycosides and Apigenin-C-Glycosides[J]. Science and Technology of Food Industry, 2022, 43(13): 345−352. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100016.

芹菜素黄酮氧苷、黄酮碳苷同分异构体抗炎活性评价

Anti-inflammation Activity Evaluation of Apigenin-O-Glycosides and Apigenin-C-Glycosides

  • 摘要: 目的:评价2种芹菜素黄酮氧苷(芹菜素-7-O-β-D-吡喃葡萄糖苷和5-O-β-D-吡喃葡萄糖苷芹菜甙元)和2种芹菜素黄酮碳苷(牡荆素和异牡荆素)的抗炎活性。方法:采用Western Blot法测定芹菜素黄酮苷在炎性细胞THP-1中的蛋白变化,采用酶联免疫法和实时荧光定量聚合酶链式反应法分析细胞中细胞因子变化。结果:4种芹菜素黄酮苷均能极显著抑制IL-10 mRNA的上升(P<0.01),且牡荆素、异牡荆素能够极显著地抑制环氧合酶-2酶活力(P<0.01),此外,异牡荆素还通过极显著抑制白细胞介素1βP<0.01)和白细胞介素6(P<0.01)发挥了更有效的抗炎作用,而芹菜素-7-O-β-D-吡喃葡萄糖苷极显著抑制了肿瘤怀素因子TNF-α的表达(P<0.01),5-O-β-D-吡喃葡萄糖苷芹菜甙元则对除IL-10外的其余几种细胞因子无抑制作用;进一步的研究发现,8位碳苷取代形成的芹菜素黄酮苷(即异牡荆素)对核转录因子P65(核转录因子κB(nuclear factor κB,NF-κB)亚基之一)的核移位抑制作用显著强于其余3种芹菜素黄酮苷的抑制作用(P<0.01)。但异牡荆素未通过抑制上游调控蛋白IκBαα Inhibitor of κB,κB抑制因子α亚基)、IKKβ(IκB Kinase β,IκB激酶β亚基)而抑制NF-κB蛋白家族P65蛋白。结论:芹菜素黄酮碳苷尤其是异牡荆素在THP-1炎性细胞模型中具有更好的抗炎活性作用,但异牡荆素的抗炎作用并非通过IκBα依赖型的NF-κB信号通路实现。

     

    Abstract: Objective: The anti-inflammatory activities of apigenin-7-O-β-D-glucopyranoside, apigenin 5-O-glucoside, vitexin and isovitexin were evaluated. Methods: Changes of protein in THP-1 cells pretreated with flavonoids were measured by Western blot test. The differences of cytokines were analyzed by enzyme-linked immunosorbent assay and fluorescence quantitative polymerase chain reaction. Results: It showed that all the flavonoids tested could repress the gene expression of IL-10 (P<0.01). Vitexin and isovitexin inhibited the production of cyclooxygenase-2 as well (P<0.01). What’s more, isovitexin significantly suppressed the increases of interleukin-1β (P<0.01) and interleukin-6 (P<0.01). Apigenin-7-O-β-D-glucopyranoside only significantly inhibited TNF-α (P<0.01). Apart from IL-10 mRNA, apigenin 5-O-glucoside displayed no anti-inflammatory effects according to the other results of cytokine analyses. Furthermore, the structure of apigenin-C-glycoside substituted at position 8, namely isovitexin showed the strongest inhibition on nuclear translocation of P65 (P<0.01). However, this suppression effect of isovitexin on P65 was not through inhibiting upstream regulation proteins such as IκBα and IKKβ. Conclusion: In a word, compared with apigenin-O-glycosides, apigenin-C-glycosides especially isovitexin had a better anti-inflammatory activity based on cell line THP-1. But the anti-inflamamtion of isovitexin was not IκBα -dependent NF- κB signal pathway.

     

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