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中国精品科技期刊2020
谭婉清,王术玲,关日晴. HPLC法测定保健食品B族维生素片中9种维生素B的含量[J]. 食品工业科技,2022,43(13):303−310. doi: 10.13386/j.issn1002-0306.2021110019.
引用本文: 谭婉清,王术玲,关日晴. HPLC法测定保健食品B族维生素片中9种维生素B的含量[J]. 食品工业科技,2022,43(13):303−310. doi: 10.13386/j.issn1002-0306.2021110019.
TAN Wanqing, WANG Shuling, GUAN Riqing. Determination of Nine Kinds of Vitamin B Components in Healthy Food Vitamin B Tablets by HPLC[J]. Science and Technology of Food Industry, 2022, 43(13): 303−310. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110019.
Citation: TAN Wanqing, WANG Shuling, GUAN Riqing. Determination of Nine Kinds of Vitamin B Components in Healthy Food Vitamin B Tablets by HPLC[J]. Science and Technology of Food Industry, 2022, 43(13): 303−310. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110019.

HPLC法测定保健食品B族维生素片中9种维生素B的含量

Determination of Nine Kinds of Vitamin B Components in Healthy Food Vitamin B Tablets by HPLC

  • 摘要: 目的:建立高效液相色谱-二极管阵列检测(HPLC-DAD)法测定B族维生素片中9种维生素B成分的含量。方法:样品中的叶酸经0.3%氨溶液超声提取,样品中的生物素、维生素B12和泛酸经水超声提取,样品中的维生素B1、维生素B2、维生素B6、烟酸和烟酰胺经0.1%盐酸超声提取,经C18柱分离,以甲醇-0.1 mol/L磷酸二氢钾溶液为流动相进行梯度洗脱,泛酸和生物素在205 nm波长处进行检测,维生素B1、维生素B2、烟酸和烟酰胺在256 nm波长处进行检测,维生素B6和叶酸在287 nm波长处进行检测,维生素B12在361 nm波长处进行检测。结果:9种成分能实现较好地分离,在各自线性范围内线性关系良好,决定系数R2均大于0.999,平均回收率为91.8%~106.9%,RSD为0.2%~4.8%,方法的检出限在0.05~4.7 mg/kg之间。结论:该方法操作快速简便、精密度和重复性好,能快速完成保健食品B族维生素片中9种维生素B成分的测定,并降低成本。

     

    Abstract: Objective: To establish a method for the determination of nine vitamin B components in vitamin B tablets by high performance liquid chromatography-diode array detector (HPLC-DAD). Method: Folic acid was ultrasonically extracted with 0.3% ammonia solution, biotin, vitamin B12 and pantothenate acid were ultrasonically extracted with water, the vitamins B1, B2, B6, niacin and nicotinamide were ultrasonically extracted by 0.1% hydrochloric acid, separated by C18 column with methanol-0.1 mol/L potassium dihydrogen phosphate solution as the mobile phase for gradient elution. Pantothenate acid and biotin were detected at the wavelength of 205 nm, vitamins B1, B2, niacin and nicotinamide were detected at the wavelength of 256 nm, vitamins B6 and folic acid were detected at the wavelength of 287 nm, vitamins B12 was detected at the wavelength of 361 nm. Results: The results showed that the nine components could be separated well, and the linear relationship was good in their linear range. The average recoveries were 91.8%~106.9%, RSD were 0.2%~4.8%, and the detection limit of the method were 0.05~4.7 mg/kg. Conclusion: This method is rapid, simple, accurate and repeatable. It can quickly complete the determination of nine vitamin B components in health food vitamin B tablets and reduce the cost.

     

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