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中国精品科技期刊2020
童凡,黄家琪,范坚强,等. 常压室温等离子体诱变选育淀粉酶菌株及其酶学特性研究[J]. 食品工业科技,2022,43(20):137−143. doi: 10.13386/j.issn1002-0306.2021120194.
引用本文: 童凡,黄家琪,范坚强,等. 常压室温等离子体诱变选育淀粉酶菌株及其酶学特性研究[J]. 食品工业科技,2022,43(20):137−143. doi: 10.13386/j.issn1002-0306.2021120194.
TONG Fan, HUANG Jiaqi, FAN Jianqiang, et al. Selection of Amylase Producing Strain by Atmospheric and Room Temperature Plasmas and Its Enzymological Properties[J]. Science and Technology of Food Industry, 2022, 43(20): 137−143. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120194.
Citation: TONG Fan, HUANG Jiaqi, FAN Jianqiang, et al. Selection of Amylase Producing Strain by Atmospheric and Room Temperature Plasmas and Its Enzymological Properties[J]. Science and Technology of Food Industry, 2022, 43(20): 137−143. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120194.

常压室温等离子体诱变选育淀粉酶菌株及其酶学特性研究

Selection of Amylase Producing Strain by Atmospheric and Room Temperature Plasmas and Its Enzymological Properties

  • 摘要: 目的:以云南马龙C3F-2016烟叶表面筛选的产淀粉酶菌株为出发菌株,诱变选育高产淀粉酶菌株并研究诱变前后酶学特性。方法:ARTP技术选育菌株后采用3,5-二硝基水杨酸法测定诱变前后菌株产生的淀粉酶酶活力,并探讨不同温度、不同pH、金属离子、紫外光对淀粉酶活力的影响。结果:诱变选育出一株酶活力有较大提高且传代稳定的突变株Bacillus koreensis FS-103,其淀粉酶活力达9050 U/mL,较出发菌株提高了90%。高产突变株FS-103所产淀粉酶的最适作用温度为60 ℃,最适作用pH5.5,在40~50 ℃和pH5~6之间具有良好的稳定性,Ca2+、Mg2+对淀粉酶活性具有促进作用,紫外照射对淀粉酶活力影响减弱。结论:此诱变选育产淀粉酶菌株的方法可行,为该类菌株进行酶制剂的研究与开发奠定理论基础。

     

    Abstract: Objective: The amylase-producing strain screened on the surface of Yunnan Malong C3F-2016 tobacco leaves were used as the starting strain to mutagenically select high amylase-producing strain and to study the enzymatic properties before and after mutagenesis. Methods: The amylase-producing strain were selected by ARTP technique and the amylase activity was determined by the 3,5-dinitrosalicylic acid (DNS) method before and after mutagenesis, and the effects of different temperatures, pH, metal ions and UV light on the amylase activity were investigated. Results: A mutant strain Bacillus koreensis FS-103 was selected by mutagenesis with improved enzyme activity and stable transmission, and its amylase activity reached 9050 U/mL, which was 90% higher than that of the starting strain. The optimum action temperature of amylase produced by the high-yielding mutant strain FS-103 was 60 ℃ and the optimum action pH5.5, with good stability between 40 and 50 ℃ and pH5~6, Ca2+ and Mg2+ had a promoting effect on amylase activity, and the effect of UV irradiation on amylase activity was weakened. Conclusion: This method of mutagenic selection of amylase-producing strain was feasible and lays the theoretical foundation for the research and development of enzyme preparations by this type of strain.

     

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