• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
关玉婷,温思萌,冯雪,等. 茯苓渣多糖组成分析及体外抗癌、免疫活性研究[J]. 食品工业科技,2022,43(21):381−387. doi: 10.13386/j.issn1002-0306.2022010168.
引用本文: 关玉婷,温思萌,冯雪,等. 茯苓渣多糖组成分析及体外抗癌、免疫活性研究[J]. 食品工业科技,2022,43(21):381−387. doi: 10.13386/j.issn1002-0306.2022010168.
GUAN Yuting, WEN Simeng, FENG Xue, et al. Composition Analysis, Anticancer and Immune Activities of Polysaccharides from Poria cocos Residues in Vitro[J]. Science and Technology of Food Industry, 2022, 43(21): 381−387. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022010168.
Citation: GUAN Yuting, WEN Simeng, FENG Xue, et al. Composition Analysis, Anticancer and Immune Activities of Polysaccharides from Poria cocos Residues in Vitro[J]. Science and Technology of Food Industry, 2022, 43(21): 381−387. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022010168.

茯苓渣多糖组成分析及体外抗癌、免疫活性研究

Composition Analysis, Anticancer and Immune Activities of Polysaccharides from Poria cocos Residues in Vitro

  • 摘要: 目的:以茯苓渣为原料,通过热水浸提茯苓多糖,研究其单糖组成及抗癌、抗炎症因子及免疫活性,为开发茯苓多糖相关产品提供了理论依据。方法:利用高效液相色谱和红外光谱对水提茯苓多糖进行单糖组成分析,采用CCK-8法对水提茯苓多糖进行抗肿瘤及免疫活性研究。结果:水提茯苓多糖的多糖含量为76%,具有明显的多糖特征吸收峰,主要由葡萄糖、甘露糖、半乳糖、岩藻糖组成,其物质的量之比为1:0.107:0.079:0.018;对胃癌细胞、乳腺癌细胞、肝癌细胞均有抑制作用,对胃癌细胞的增殖抑制效果最优(IC50=1096 µg/mL),对小鼠脾淋巴细胞具有促进增殖作用。结论:水提茯苓多糖具有良好的体外抗癌及免疫活性。

     

    Abstract: Objective: The monosaccharide composition of Poria cocos polysaccharide (PCOS) from Poria cocos residue extracted by hot water, as well as its anti-cancer, anti-inflammatory factors and immune activities were studied, providing a theoretical foundation for the development of PCOS related products. Methods: The monosaccharide composition of water-extracted PCOS was analyzed by HPLC and FTIR, and the anti-tumor and immune activities of water-extracted PCOS were studied by CCK-8 method. Results: The polysaccharide content of PCOS was 76%, mainly composed of glucose, mannose, galactose and fucose, and the molar ratio was 1:0.107:0.079:0.018, with obvious polysaccharide characteristic absorption peak. The proliferation of gastric cancer cells, breast cancer cells, and liver cancer cells was decreased, with the best inhibition impact on gastric cancer cells (IC50=1096 µg/mL), and spleen lymphocyte proliferation in mice was enhanced. Conclusion: The water-extracted PCOS has good anti-cancer and immune activities in vitro.

     

/

返回文章
返回